Evidence map›Paper›PMID 24964161›Full record

ArticlePloS one2014

Biological role of Trichoderma harzianum-derived platelet-activating factor acetylhydrolase (PAF-AH) on stress response and antagonism.

Chuanjin Yu, Lili Fan, Qiong Wu, Kehe Fu, Shigang Gao, Meng Wang, Jinxin Gao, Yaqian Li, Jie Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
3.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Chuanjin YuDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Lili FanDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Qiong WuDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Kehe FuDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Shigang GaoDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Meng WangDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Jinxin GaoDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Yaqian LiDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Jie ChenDepartment of Resource and Environmental Science, School of Agriculture and Biology, Shanghai Jiao tong University, Shanghai, P. R. China; State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P. R. China.
Shanghai Jiao Tong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We investigated the properties of platelet-activating factor acetylhydrolase (PAF-AH) derived from Trichoderma harzianum. The enzyme, comprised of 572 amino acids, shares high homology with PAF-AH proteins from T. koningii and other microbial species. The optimum enzymatic activity of PAF-AH occurred at pH 6 in the absence of Ca2+ and it localized in the cytoplasm, and we observed the upregulation of PAF-AH expression in response to carbon starvation and strong heat shock. Furthermore, PAF-AH knockout transformant growth occurred more slowly than wild type cells and over-expression strains grown in SM medium at 37°C and 42°C. In addition, PAF-AH expression significantly increased under a series of maize root induction assay. Eicosanoic acid and ergosterol levels decreased in the PAF-AH knockouts compared to wild type cells, as revealed by GC/MS analysis. We also determined stress responses mediated by PAF-AH were related to proteins HEX1, Cu/Zn superoxide dismutase, and cytochrome c. Finally, PAF-AH exhibited antagonistic activity against Rhizoctonia solani in plate confrontation assays. Our results indicate PAF-AH may play an important role in T. harzianum stress response and antagonism under diverse environmental conditions.

Indexed as

Stress, Physiological1-Alkyl-2-acetylglycerophosphocholine EsteraseCloning, MolecularFatty AcidsGene Knockout TechniquesIntracellular SpaceProtein TransportRhizoctoniaSterolsTransformation, GeneticTrichoderma1-Alkyl-2-acetylglycerophosphocholine EsteraseFatty AcidsSterols

Identifiers

PMID24964161
PMCPMC4070952
OpenAlexW1975458575

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.