ArticleProtein science : a publication of the Protein Society2014
An osmolyte mitigates the destabilizing effect of protein crowding.
Article in Protein science : a publication of the Protein Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Using NMR-detected hydrogen-deuterium exchange to quantify protein stability in cosolutes, under crowded conditionsMagnetic resonance letters · 2023Review
- When Phased without Water: Biophysics of Cellular Desiccation, from Biomolecules to Condensates.Chemical reviews · 2023Review
- Improving L-threonine production in Escherichia coli by elimination of transporters ProP and ProVWX.Microbial cell factories · 2021Article
- Protecting activity of desiccated enzymes.Protein science : a publication of the Protein Society · 2019Article
- Conditionally disordered proteins: bringing the environment back into the fold.Cellular and molecular life sciences : CMLS · 2017Review
- Crowding in Cellular Environments at an Atomistic Level from Computer Simulations.The journal of physical chemistry. B · 2017Article
- Cosolutes, Crowding, and Protein Folding Kinetics.The journal of physical chemistry. B · 2017Article
- Microorganisms maintain crowding homeostasis.Nature reviews. Microbiology · 2017Review
- Osmotic Shock Induced Protein Destabilization in Living Cells and Its Reversal by Glycine Betaine.Journal of molecular biology · 2017Article
- Thermodynamics and solvent linkage of macromolecule-ligand interactions.Methods (San Diego, Calif.) · 2015Review
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2 authors.
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Abstract
Most theories predict that macromolecular crowding stabilizes globular proteins, but recent studies show that weak attractive interactions can result in crowding-induced destabilization. Osmolytes are ubiquitous in biology and help protect cells against stress. Given that dehydration stress adds to the crowded nature of the cytoplasm, we speculated that cells might use osmolytes to overcome the destabilization caused by the increased weak interactions that accompany desiccation. We used NMR-detected amide proton exchange experiments to measure the stability of the test protein chymotrypsin inhibitor 2 under physiologically relevant crowded conditions in the presence and absence of the osmolyte glycine betaine. The osmolyte overcame the destabilizing effect of the cytosol. This result provides a physiologically relevant explanation for the accumulation of osmolytes by dehydration-stressed cells.
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