ArticlePloS one2014
Synthetic promoter library for modulation of actinorhodin production in Streptomyces coelicolor A3(2).
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Design and Testing of Root-Specific Synthetic Promoters by Machine Learning.International journal of molecular sciences · 2026Article
- CRISPR/Cas9-Mediated Promoter Engineering in Saccharopolyspora erythraea.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The roles of SARP family regulators involved in secondary metabolism inFrontiers in microbiology · 2024Review
- Exploring and Engineering Novel Strong Promoters for High-Level Protein Expression inMicroorganisms · 2023Article
- A universal approach to gene expression engineering.Synthetic biology (Oxford, England) · 2022Article
- High-Throughput Transcriptional Characterization of Regulatory Sequences from Bacterial Biosynthetic Gene Clusters.ACS synthetic biology · 2021Article
- Identification of a gene from Streptomyces rimosus M527 negatively affecting rimocidin biosynthesis and morphological differentiation.Applied microbiology and biotechnology · 2020Article
- Coordinated regulation for nature products discovery and overproduction inSynthetic and systems biotechnology · 2020Article
- Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters.Plant biotechnology journal · 2020Article
- Modular and Integrative Vectors for Synthetic Biology Applications inApplied and environmental microbiology · 2019Article
- Selection of an efficient promoter and its application in toyocamycin production improvement in Streptomyces diastatochromogenes 1628.World journal of microbiology & biotechnology · 2017Article
- Biofilm as a production platform for heterologous production of rhamnolipids by the non-pathogenic strain Pseudomonas putida KT2440.Microbial cell factories · 2016Article
- Characterization of a pathway-specific activator of milbemycin biosynthesis and improved milbemycin production by its overexpression in Streptomyces bingchenggensis.Microbial cell factories · 2016Article
- Synthetic promoter design for new microbial chassis.Biochemical Society transactions · 2016Review
- Synthetic biology to access and expand nature's chemical diversity.Nature reviews. Microbiology · 2016Review
- Novel green tissue-specific synthetic promoters and cis-regulatory elements in rice.Scientific reports · 2015Article
- Identification of a cluster-situated activator of oxytetracycline biosynthesis and manipulation of its expression for improved oxytetracycline production in Streptomyces rimosus.Microbial cell factories · 2015Article
- Unraveling the kinetic diversity of microbial 3-dehydroquinate dehydratases of shikimate pathway.AMB Express · 2015Article
- Development and optimization of an intergeneric conjugation system and analysis of promoter activity in Streptomyces rimosus M527.Journal of Zhejiang University. Science. BArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was the application of the synthetic promoter library (SPL) technology for modulation of actinorhodin production in Streptomyces coelicolor A3(2). The SPL technology was used to optimize the expression of a pathway specific positive transcriptional regulator ActII orf4, which activates the transcription of the S. coelicolor actinorhodin biosynthetic gene cluster. The native actII orf4 promoter was replaced with synthetic promoters, generating a S. coelicolor library with a broad range of expression levels of actII orf4. The resulting library was screened based on the yield of actinorhodin. Selected strains were further physiologically characterized. One of the strains from the library, ScoSPL20, showed considerably higher yield of actinorhodin and final actinorhodin titer, compared to S. coelicolor wild type and S. coelicolor with actII orf4 expressed from a strong constitutive promoter. ScoSPL20 demonstrated exceptional productivity despite having a comparatively weak expression from the promoter. Interestingly, the ScoSPL20 promoter was activated at a much earlier stage of growth compared to the wild type, demonstrating the advantage of fine-tuning and temporal tuning of gene expression in metabolic engineering. Transcriptome studies were performed in exponential and actinorhodin-producing phase of growth to compare gene expression between ScoSPL20 and the wild type. To our knowledge, this is the first successful application of the SPL technology for secondary metabolite production in filamentous bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.