ArticlePloS one2014
Development of loop-mediated isothermal amplification (LAMP) assay for rapid and sensitive identification of ostrich meat.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment.Journal of agricultural and food chemistry · 2024Article
- Rapid Detection of Hepatitis A Virus in Foods Using a Bioluminescent Assay in Real-Time (BART) and Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) Technology.Food and environmental virology · 2023Article
- Real-Time Portable LAMP Assay for a Rapid Detection of Xylella fastidiosa In-Field.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Loop-Mediated Isothermal Amplification (LAMP) and SYBR Green qPCR for Fast and Reliable Detection ofPlants (Basel, Switzerland) · 2022Article
- Article
- Identification of sheep (Journal of food science and technology · 2021Article
- Chemometrics in Tandem with Hyperspectral Imaging for Detecting Authentication of Raw and Cooked Mutton Rolls.Foods (Basel, Switzerland) · 2021Article
- Review
- Rapid diagnostics for Gnomoniopsis smithogilvyi (syn. Gnomoniopsis castaneae) in chestnut nuts: new challenges by using LAMP and real-time PCR methods.AMB Express · 2021Article
- Rapid Detection ofPlants (Basel, Switzerland) · 2021Article
- Development of a loop-mediated isothermal amplification (LAMP) assay for the identification of the invasive wood borer3 Biotech · 2021Article
- Article
- Specific, sensitive and rapid authentication of donkey-hide gelatine (Journal of food science and technology · 2020Article
- Real-time loop-mediated isothermal amplification: an early-warning tool for quarantine plant pathogen detection.AMB Express · 2019Article
- Article
- The rapid and sensitive detection of edible bird's nest (Aerodramus fuciphagus) in processed food by a loop-mediated isothermal amplification (LAMP) assay.Journal of food and drug analysis · 2019Article
- The development of loop-mediated isothermal amplification (LAMP) assays for the rapid authentication of five forbidden vegetables in strict vegetarian diets.Scientific reports · 2017Article
- Article
- LAMP assay for rapid diagnosis of cow DNA in goat milk and meat samples.Iranian journal of veterinary research · 2017Article
- Application of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Cow Components Adulterated in Buffalo Milk/Meat.Molecular biotechnology · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Animal species identification is one of the primary duties of official food control. Since ostrich meat is difficult to be differentiated macroscopically from beef, therefore new analytical methods are needed. To enforce labeling regulations for the authentication of ostrich meat, it might be of importance to develop and evaluate a rapid and reliable assay. In the present study, a loop-mediated isothermal amplification (LAMP) assay based on the cytochrome b gene of the mitochondrial DNA of the species Struthio camelus was developed. The LAMP assay was used in combination with a real-time fluorometer. The developed system allowed the detection of 0.01% ostrich meat products. In parallel, a direct swab method without nucleic acid extraction using the HYPLEX LPTV buffer was also evaluated. This rapid processing method allowed detection of ostrich meat without major incubation steps. In summary, the LAMP assay had excellent sensitivity and specificity for detecting ostrich meat and could provide a sampling-to-result identification-time of 15 to 20 minutes.
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