ArticleJournal of visualized experiments : JoVE2014
Automated analysis of dynamic Ca2+ signals in image sequences.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Automated ROI detection allows rapid quantification of synaptic activity across tens of thousands of synapses in cell culture.Frontiers in synaptic neuroscience · 2026Article
- Hyperspectral imaging and dynamic region of interest tracking approaches to quantify localized cAMP signals.Biochemical Society transactions · 2024Review
- Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening.Communications biology · 2023Article
- Article
- In Vitro Model to Investigate Communication between Dorsal Root Ganglion and Spinal Cord Glia.International journal of molecular sciences · 2021Article
- Long-Term Hypoxia Negatively Influences CaFrontiers in physiology · 2021Article
- Direct and Intervertebral Disc-Mediated Sensitization of Dorsal Root Ganglion Neurons by Hypoxia and Low pH.Neurospine · 2020Article
- Simulation of calcium signaling in fine astrocytic processes: Effect of spatial properties on spontaneous activity.PLoS computational biology · 2019Article
- Functional Interaction among KInternational journal of molecular sciences · 2019Review
- Hyperspectral imaging fluorescence excitation scanning spectral characteristics of remodeled mouse arteries.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Long-Term High-Altitude Hypoxia and Alpha Adrenoceptor-Dependent Pulmonary Arterial Contractions in Fetal and Adult Sheep.Frontiers in physiology · 2019Article
- Changes in vascular reactivity and endothelial CaMicrocirculation (New York, N.Y. : 1994) · 2017Article
- Endothelial SK3 channel-associated Ca2+ microdomains modulate blood pressure.American journal of physiology. Heart and circulatory physiology · 2016Article
- No Static at All: Tuning Into the Complexities of Ca2+ Signaling in the Endothelium.Circulation research · 2016Article
- Decoding dynamic Ca(2+) signaling in the vascular endothelium.Frontiers in physiology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Intracellular Ca(2+) signals are commonly studied with fluorescent Ca(2+) indicator dyes and microscopy techniques. However, quantitative analysis of Ca(2+) imaging data is time consuming and subject to bias. Automated signal analysis algorithms based on region of interest (ROI) detection have been implemented for one-dimensional line scan measurements, but there is no current algorithm which integrates optimized identification and analysis of ROIs in two-dimensional image sequences. Here an algorithm for rapid acquisition and analysis of ROIs in image sequences is described. It utilizes ellipses fit to noise filtered signals in order to determine optimal ROI placement, and computes Ca(2+) signal parameters of amplitude, duration and spatial spread. This algorithm was implemented as a freely available plugin for ImageJ (NIH) software. Together with analysis scripts written for the open source statistical processing software R, this approach provides a high-capacity pipeline for performing quick statistical analysis of experimental output. The authors suggest that use of this analysis protocol will lead to a more complete and unbiased characterization of physiologic Ca(2+) signaling.
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Registered trials
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