ArticleInternational journal of medical microbiology : IJMM2014
Identification of genes induced in Vibrio cholerae in a dynamic biofilm system.
Article in International journal of medical microbiology : IJMM, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Negative feedback of cyclic di-GMP levels optimizes switching between sessile and motile lifestyles inbioRxiv : the preprint server for biology · 2024Article
- The activity of the quorum sensing regulator HapR is modulated by the bacterial extracellular vesicle (BEV)-associated protein ObfA of Vibrio cholerae.Journal of extracellular vesicles · 2024Article
- Advances in cholera research: from molecular biology to public health initiatives.Frontiers in microbiology · 2023Review
- Impact of Gene Repression on Biofilm Formation ofFrontiers in microbiology · 2022Article
- Comparison of TwoMicroorganisms · 2021Article
- TetR RegulatedBio-protocol · 2020Article
- Virulence Factors Produced byMolecular & cellular proteomics : MCP · 2019Article
- A Broad Spectrum Protein Glycosylation System Influences Type II Protein Secretion and Associated Phenotypes inFrontiers in microbiology · 2019Article
- Silence is golden: gene silencing of V. cholerae during intestinal colonization delivers new aspects to the acid tolerance response.Gut microbes · 2019Article
- A Perspective on the Global Pandemic of Waterborne Disease.Microbial ecology · 2018Article
- In vivo repressed genes ofProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Vibrio cholerae Combines Individual and Collective Sensing to Trigger Biofilm Dispersal.Current biology : CB · 2017Article
- ToxR Antagonizes H-NS Regulation of Horizontally Acquired Genes to Drive Host Colonization.PLoS pathogens · 2016Article
- Vibrio cholerae Biofilms and Cholera Pathogenesis.PLoS neglected tropical diseases · 2016Review
- Optical Sensing of Microbial Life on Surfaces.Applied and environmental microbiology · 2015Review
- Genes Activated bymSphereArticle
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
The facultative human pathogen Vibrio cholerae, the causative agent of the severe secretory diarrheal disease cholera, persists in its aquatic reservoirs in biofilms during interepidemic periods. Biofilm is a likely form in which clinically relevant V. cholerae is taken up by humans, providing an infective dose. Thus, a better understanding of biofilm formation of V. cholerae is relevant for the ecology and epidemiology of cholera as well as a target to control the disease. Most previous studies have investigated static biofilms of V. cholerae and elucidated structural prerequisites like flagella, pili and a biofilm matrix including extracellular DNA, numerous matrix proteins and exopolysaccharide, as well as the involvement of regulatory pathways like two-component systems, quorum sensing and c-di-GMP signaling. However, aquatic environments are more likely to reflect an open, dynamic system. Hence, we used a biofilm system with constant medium flow and a temporal controlled reporter-system of transcription to identify genes induced during dynamic biofilm formation. We identified genes known or predicted to be involved in c-di-GMP signaling, motility and chemotaxis, metabolism, and transport. Subsequent phenotypic characterization of mutants with independent mutations in candidate dynamic biofilm-induced genes revealed novel insights into the physiology of static and dynamic biofilm conditions. The results of this study also reinforce the hypotheses that distinct differences in regulatory mechanisms governing biofilm development are present under dynamic conditions compared to static conditions.
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Registered trials
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