ArticleJournal of visualized experiments : JoVE2014
Combined DNA-RNA fluorescent in situ hybridization (FISH) to study X chromosome inactivation in differentiated female mouse embryonic stem cells.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- Modern RNA Quantification Methods: From RT-qPCR to Advanced Microscopy.The journal of physical chemistry. B · 2026Review
- Allele-level visualization of transcription and chromatin by high-throughput imaging.Histochemistry and cell biology · 2024Article
- Allele-level visualization of transcription and chromatin by high-throughput imaging.Research square · 2024Article
- Allele-level visualization of transcription and chromatin by high-throughput imaging.bioRxiv : the preprint server for biology · 2024Article
- LncRNA Osilr9 coordinates promoter DNA demethylation and the intrachromosomal loop structure required for maintaining stem cell pluripotency.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Novel Approaches to Profile Functional Long Noncoding RNAs Associated with Stem Cell Pluripotency.Current genomics · 2020Review
- Article
- Cytoplasmic DNA can be detected by RNA fluorescence in situ hybridization.Nucleic acids research · 2019Article
- Profiling the long noncoding RNA interaction network in the regulatory elements of target genes by chromatin in situ reverse transcription sequencing.Genome research · 2019Article
- Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments.Journal of visualized experiments : JoVE · 2017Article
- Round Spermatid Injection Rescues Female Lethality of a Paternally Inherited Xist Deletion in Mouse.PLoS genetics · 2016Article
- Dynamics of gene silencing during X inactivation using allele-specific RNA-seq.Genome biology · 2015Article
- Stable X chromosome reactivation in female human induced pluripotent stem cells.Stem cell reports · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent in situ hybridization (FISH) is a molecular technique which enables the detection of nucleic acids in cells. DNA FISH is often used in cytogenetics and cancer diagnostics, and can detect aberrations of the genome, which often has important clinical implications. RNA FISH can be used to detect RNA molecules in cells and has provided important insights in regulation of gene expression. Combining DNA and RNA FISH within the same cell is technically challenging, as conditions suitable for DNA FISH might be too harsh for fragile, single stranded RNA molecules. We here present an easily applicable protocol which enables the combined, simultaneous detection of Xist RNA and DNA encoded by the X chromosomes. This combined DNA-RNA FISH protocol can likely be applied to other systems where both RNA and DNA need to be detected.
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Registered trials
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