ArticleJournal of the renin-angiotensin-aldosterone system : JRAAS2015
Nuclear expression of renin-angiotensin system components in NRK-52E renal epithelial cells.
Article in Journal of the renin-angiotensin-aldosterone system : JRAAS, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Evidence that angiotensin II does not directly stimulate the MD2-TLR4 innate inflammatory pathway.Peptides · 2021Article
- Mas receptor is translocated to the nucleus upon agonist stimulation in brainstem neurons from spontaneously hypertensive rats but not normotensive rats.Cardiovascular research · 2020Article
- Angiotensin Receptors Heterodimerization and Trafficking: How Much Do They Influence Their Biological Function?Frontiers in pharmacology · 2020Review
- Inhibition of STAT3 activation mediated by toll-like receptor 4 attenuates angiotensin II-induced renal fibrosis and dysfunction.British journal of pharmacology · 2019Article
- Renin angiotensin aldosterone inhibition in the treatment of cardiovascular disease.Pharmacological research · 2017Review
- Angiotensinogen import in isolated proximal tubules: evidence for mitochondrial trafficking and uptake.American journal of physiology. Renal physiology · 2017Article
- Evidence for a mitochondrial angiotensin-(1-7) system in the kidney.American journal of physiology. Renal physiology · 2016Article
- Biochemical evaluation of the renin-angiotensin system: the good, bad, and absolute?American journal of physiology. Heart and circulatory physiology · 2016Review
- Age-Related Macular Degeneration and Intracrine Biology: An Hypothesis.Ochsner journal · 2016Review
- Intracellular angiotensin (1-7) increases the inward calcium current in cardiomyocytes. On the role of PKA activation.Molecular and cellular biochemistry · 2015Article
- Chemical Communication between Heart Cells is Disrupted by Intracellular Renin and Angiotensin II: Implications for Heart Development and Disease.Frontiers in endocrinology · 2015Review
- Angiotensin-(1-7) abolishes AGE-induced cellular hypertrophy and myofibroblast transformation via inhibition of ERK1/2.Cellular signalling · 2014Article
- The ins and outs of angiotensin processing within the kidney.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Review
- Evidence for an angiotensin-(1-7) neuropeptidase expressed in the brain medulla and CSF of sheep.Journal of neurochemistry · 2014Article
- Update on the Angiotensin converting enzyme 2-Angiotensin (1-7)-MAS receptor axis: fetal programing, sex differences, and intracellular pathways.Frontiers in endocrinology · 2014Review
- Intracellular Renin Disrupts Chemical Communication between Heart Cells. Pathophysiological Implications.Frontiers in endocrinology · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
introductionIsolated nuclei of sheep proximal tubules express angiotensin (Ang) receptors as well as angiotensinogen (AGT) and renin. The present study characterized the NRK-52E tubular epithelial cell line for the intracellular expression of renin-angiotensin system (RAS) components.
methodsRAS components were visualized by immunofluorescent staining in intact cells and protein expression in isolated nuclei.
resultsAn antibody to the angiotensin I (Ang I) sequence of AGT (AI-AGT) revealed only cytosolic staining, while an antibody to an internal sequence of AGT (Int-AGT) revealed primarily nuclear staining. Immunoblots of nuclear and cytosolic fractions confirmed the differential cell staining of AGT. Immunostaining for renin was present on nuclei of intact cells. Nuclear renin activity averaged 0.77±0.05 nmol/mg protein/h that was reduced by aliskiren (0.13±0.01 nmol/mg/h, n=3, p<0.01); trypsin activation increased activity three-fold. Peptide staining localized angiotensin II (Ang II) and Ang-(1-7) to the nucleus and peptide content averaged 59±2 and 57±22 fmol/mg (n=4), respectively. Peptide metabolism in isolated nuclei revealed the processing of Ang I to Ang-(1-7) by thimet oligopeptidase.
conclusionWe conclude that the NRK-52E cells express an intracellular RAS localized to the nucleus and may be an appropriate cell model to elucidate the functional relevance of this system.
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