ArticleBMC systems biology2014
Genome-scale analysis of the high-efficient protein secretion system of Aspergillus oryzae.
Article in BMC systems biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 48 citations in OpenAlex.
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- Two-Step Engineering of Food-GradeJournal of fungi (Basel, Switzerland) · 2026Article
- Transcriptomics analysis identifies folding and secretion related genes for improving monoclonal antibody production in Thermothelomyces heterothallica C1.Microbial cell factories · 2026Article
- Overexpression of msnA reprograms metabolism to enhance recombinant protein secretion across Aspergillus species.Microbial cell factories · 2026Article
- Understanding the Role of pH Regulation and Neutralizing Agents in Organic Acid Production and Growth of Aspergillus oryzae.Biotechnology and bioengineering · 2026Article
- Reconstruction of a Genome-Scale Metabolic Model forInternational journal of molecular sciences · 2025Article
- Improving recombinant protein secretion inMetabolic engineering communications · 2025Article
- Protein secretion and associated stress in industrially employed filamentous fungi.Applied microbiology and biotechnology · 2024Review
- Sub-genomic RNAi-assisted strain evolution of filamentous fungi for enhanced protein production.Applied and environmental microbiology · 2024Article
- Strategies for the Enhancement of Secondary Metabolite Production via Biosynthesis Gene Cluster Regulation inJournal of fungi (Basel, Switzerland) · 2024Review
- Edible mycelium bioengineered for enhanced nutritional value and sensory appeal using a modular synthetic biology toolkit.Nature communications · 2024Article
- Effect of the res2 transcription factor gene deletion on protein secretion and stress response in the hyperproducer strain Trichoderma reesei Rut-C30.BMC microbiology · 2023Article
- The Aspergillus fumigatus UPR is variably activated across nutrient and host environments and is critical for the establishment of corneal infection.PLoS pathogens · 2023Article
- Silencing ofJournal of fungi (Basel, Switzerland) · 2022Article
- Membrane Traffic inJournal of fungi (Basel, Switzerland) · 2021Review
- pH Changes Have a Profound Effect on Gene Expression, Hydrolytic Enzyme Production, and Dimorphism inFrontiers in microbiology · 2021Article
- Improvement of homologous GH10 xylanase production by deletion of genes with predicted function in the Aspergillus nidulans secretion pathway.Microbial biotechnology · 2020Article
- Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion.Nature communications · 2020Article
- Heterologous expression and functional characterization of the ligand-binding domain of oxysterol-binding protein from Aspergillus oryzae.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Harnessing the knowledge of protein secretion for enhanced protein production in filamentous fungi.World journal of microbiology & biotechnology · 2019Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe koji mold, Aspergillus oryzae is widely used for the production of industrial enzymes due to its particularly high protein secretion capacity and ability to perform post-translational modifications. However, systemic analysis of its secretion system is lacking, generally due to the poorly annotated proteome.
resultsHere we defined a functional protein secretory component list of A. oryzae using a previously reported secretory model of S. cerevisiae as scaffold. Additional secretory components were obtained by blast search with the functional components reported in other closely related fungal species such as Aspergillus nidulans and Aspergillus niger. To evaluate the defined component list, we performed transcriptome analysis on three α-amylase over-producing strains with varying levels of secretion capacities. Specifically, secretory components involved in the ER-associated processes (including components involved in the regulation of transport between ER and Golgi) were significantly up-regulated, with many of them never been identified for A. oryzae before. Furthermore, we defined a complete list of the putative A. oryzae secretome and monitored how it was affected by overproducing amylase.
conclusionIn combination with the transcriptome data, the most complete secretory component list and the putative secretome, we improved the systemic understanding of the secretory machinery of A. oryzae in response to high levels of protein secretion. The roles of many newly predicted secretory components were experimentally validated and the enriched component list provides a better platform for driving more mechanistic studies of the protein secretory pathway in this industrially important fungus.
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