ArticleBMC microbiology2014
Genome sequence and phenotypic analysis of a first German Francisella sp. isolate (W12-1067) not belonging to the species Francisella tularensis.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 28 citations in OpenAlex.
- Complete genome and plasmid sequence, and chemotaxonomic analysis of Francisella sp. strain W12-1067, now designated as Allofrancisella heilbronnii.BMC microbiology · 2025Article
- Detection of Francisellaceae and the differentiation of main European F. tularensis ssp. holarctica strains (Clades) by new designed qPCR assays.BMC microbiology · 2025Article
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- Molecular identification of the source of an uncommon tularaemia outbreak, Germany, autumn 2016.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019Article
- Tularemia as a waterborne disease: a review.Emerging microbes & infections · 2019Review
- Genetic Diversity and Spatial Segregation ofFrontiers in cellular and infection microbiology · 2019Article
- Construction of a New Phage Integration Vector pFIV-Val for Use in DifferentFrontiers in cellular and infection microbiology · 2018Article
- RevisitingFrontiers in microbiology · 2018Article
- Tularemia in Germany-A Re-emerging Zoonosis.Frontiers in cellular and infection microbiology · 2018Review
- Whole-Genome Relationships among Francisella Bacteria of Diverse Origins Define New Species and Provide Specific Regions for Detection.Applied and environmental microbiology · 2017Article
- Shared features of cryptic plasmids from environmental and pathogenic Francisella species.PloS one · 2017Article
- High and novel genetic diversity of Francisella tularensis in Germany and indication of environmental persistence.Epidemiology and infection · 2016Article
- Rapid Identification and Characterization of Francisella by Molecular Biology and Other Techniques.The open microbiology journal · 2016Article
- Characterization of Francisella species isolated from the cooling water of an air conditioning system.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFrancisella isolates from patients suffering from tularemia in Germany are generally strains of the species F. tularensis subsp. holarctica. To our knowledge, no other Francisella species are known for Germany. Recently, a new Francisella species could be isolated from a water reservoir of a cooling tower in Germany.
resultsWe identified a Francisella sp. (isolate W12-1067) whose 16S rDNA is 99% identical to the respective nucleotide sequence of the recently published strain F. guangzhouensis. The overall sequence identity of the fopA, gyrA, rpoA, groEL, sdhA and dnaK genes is only 89%, indicating that strain W12-1067 is not identical to F. guangzhouensis. W12-1067 was isolated from a water reservoir of a cooling tower of a hospital in Germany. The growth optimum of the isolate is approximately 30°C, it can grow in the presence of 4-5% NaCl (halotolerant) and is able to grow without additional cysteine within the medium. The strain was able to replicate within a mouse-derived macrophage-like cell line. The whole genome of the strain was sequenced (~1.7 mbp, 32.2% G + C content) and the draft genome was annotated. Various virulence genes common to the genus Francisella are present, but the Francisella pathogenicity island (FPI) is missing. However, another putative type-VI secretion system is present within the genome of strain W12-1067.
conclusionsIsolate W12-1067 is closely related to the recently described F. guangzhouensis species and it replicates within eukaryotic host cells. Since W12-1067 exhibits a putative new type-VI secretion system and F. tularensis subsp. holarctica was found not to be the sole species in Germany, the new isolate is an interesting species to be analyzed in more detail. Further research is needed to investigate the epidemiology, ecology and pathogenicity of Francisella species present in Germany.
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