ArticleBMC microbiology2014
Functional genomics to identify the factors contributing to successful persistence and global spread of an antibiotic resistance plasmid.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 38 citations in OpenAlex.
- Mechanistic insights into plasmid transfer inhibition in Enterobacterales by nucleoside analogues.npj antimicrobials and resistance · 2026Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Comparison of IncK-Antibiotics (Basel, Switzerland) · 2024Article
- Growth in a biofilm promotes conjugation of amSphere · 2023Article
- Molecular epidemiology, risk factors, and outcomes of carbapenem-resistantFrontiers in microbiology · 2023Article
- Genomic analysis of qnr-harbouring IncX plasmids and their transferability within different hosts under induced stress.BMC microbiology · 2022Article
- The Type IV Pilus of Plasmid TP114 Displays Adhesins Conferring Conjugation Specificity and Is Important for DNA Transfer in the Mouse Gut Microbiota.Microbiology spectrum · 2022Article
- Article
- Nutrition Related Stress Factors Reduce the Transfer of Extended-Spectrum Beta-Lactamase Resistance Genes between anBiomolecules · 2019Article
- Successful Host Adaptation of IncK2 Plasmids.Frontiers in microbiology · 2019Article
- Host-specific differences in the contribution of an ESBL IncI1 plasmid to intestinal colonization by Escherichia coli O104:H4.The Journal of antimicrobial chemotherapy · 2018Article
- Article
- Elucidation of quantitative structural diversity of remarkable rearrangement regions, shufflons, in IncI2 plasmids.Scientific reports · 2017Article
- Assessing the Risk of Probiotic Dietary Supplements in the Context of Antibiotic Resistance.Frontiers in microbiology · 2017Article
- Plasmids CarryingFrontiers in microbiology · 2017Article
- CDI Systems Are Stably Maintained by a Cell-Contact Mediated Surveillance Mechanism.PLoS genetics · 2016Article
- Factors that affect transfer of the IncI1 β-lactam resistance plasmid pESBL-283 between E. coli strains.PloS one · 2015Article
- Carbapenemase-producing Klebsiella pneumoniae: molecular and genetic decoding.Trends in microbiology · 2014Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe spread of bacterial plasmids is an increasing global problem contributing to the widespread dissemination of antibiotic resistance genes including β-lactamases. Our understanding of the details of the biological mechanisms by which these natural plasmids are able to persist in bacterial populations and are able to establish themselves in new hosts via conjugative transfer is very poor. We recently identified and sequenced a globally successful plasmid, pCT, conferring β-lactam resistance.
resultsHere, we investigated six plasmid encoded factors (tra and pil loci; rci shufflon recombinase, a putative sigma factor, a putative parB partitioning gene and a pndACB toxin-antitoxin system) hypothesised to contribute to the 'evolutionary success' of plasmid pCT. Using a functional genomics approach, the role of these loci was investigated by systematically inactivating each region and examining the impact on plasmid persistence, conjugation and bacterial host biology. While the tra locus was found to be essential for all pCT conjugative transfer, the second conjugation (pil) locus was found to increase conjugation frequencies in liquid media to particular bacterial host recipients (determined in part by the rci shufflon recombinase). Inactivation of the pCT pndACB system and parB did not reduce the stability of this plasmid.
conclusionsOur findings suggest the success of pCT may be due to a combination of factors including plasmid stability within a range of bacterial hosts, a lack of a fitness burden and efficient transfer rates to new bacterial hosts rather than the presence of a particular gene or phenotype transferred to the host. The methodology used in our study could be applied to other 'successful' globally distributed plasmids to discover the role of currently unknown plasmid backbone genes or to investigate other factors which allow these elements to persist and spread.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.