ArticleBlood transfusion = Trasfusione del sangue2014
Biochemical assessment of red blood cells during storage by (1)H nuclear magnetic resonance spectroscopy. Identification of a biomarker of their level of protection against oxidative stress.
Article in Blood transfusion = Trasfusione del sangue, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Trial
- Effects of red blood cell (RBC) transfusion on sickle cell disease recipient plasma and RBC metabolism.Transfusion · 2018Trial
- Article
- Analysis of purines and pyrimidines across biospecimens: influence of storage temperature and duration.Scientific reports · 2026Article
- Recommendations for sample selection, collection and preparation for NMR-based metabolomics studies of blood.Metabolomics : Official journal of the Metabolomic Society · 2025Review
- Effects of Different Ratios of Carbohydrate-Fat in Enteral Nutrition on Metabolic Pattern and Organ Damage in Burned Rats.Nutrients · 2022Article
- Proof of concept: hypoxanthine from stored red blood cells induces neutrophil activation.Blood transfusion = Trasfusione del sangue · 2022Article
- Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy.Biomolecules · 2021Article
- Article
- Article
- Nicotine exposure increases markers of oxidant stress in stored red blood cells from healthy donor volunteers.Transfusion · 2020Article
- Vesiculation of Red Blood Cells in the Blood Bank: A Multi-Omics Approach towards Identification of Causes and Consequences.Proteomes · 2020Article
- Patient Blood Management: transfusion appropriateness in the post-operative period.Blood transfusion = Trasfusione del sangue · 2019Observational
- Effects of aged stored autologous red blood cells on human plasma metabolome.Blood advances · 2019Article
- Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS.Molecules (Basel, Switzerland) · 2018Article
- Hypoxia modulates the purine salvage pathway and decreases red blood cell and supernatant levels of hypoxanthine during refrigerated storage.Haematologica · 2018Article
- Comparative Evaluation of Biochemical and Hematological Parameters of Pre-Storage Leukoreduction during RBC Storage.International journal of hematology-oncology and stem cell research · 2018Article
- Mesenchymal stromal cells can be applied to red blood cells storage as a kind of cellular additive.Bioscience reports · 2017Article
- A three-minute method for high-throughput quantitative metabolomics and quantitative tracing experiments of central carbon and nitrogen pathways.Rapid communications in mass spectrometry : RCM · 2017Article
- Whole Blood Metabolomics byAnalytical chemistry · 2017Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBlood transfusion is an established therapeutic practice. The characteristics of blood components at different storage times are expected to affect the efficacy of transfusion therapy. Metabolic profiling by nuclear magnetic resonance (NMR) spectroscopy requires little or no sample treatment and allows identification of more than 50 soluble metabolites in a single experiment. The aim of this study was to assess the metabolic behaviour of red blood cells during 42 days of storage in blood bank conditions. MATERIALS AND
methodsRed blood cells (RBC), collected from eight healthy male donors, aged 25-50 years, were prepared as prestorage leukoreduced erythrocyte concentrates and stored under standard blood bank conditions. Samples taken at various storage times were separated in two fractions: the supernatant, recovered after centrifugation, and the red blood cell lysate obtained after protein depletion by ultrafiltration. The metabolic profile of the red blood cells was determined from analysis of (1)H-NMR spectra.
resultsThe red blood cell supernatant was studied to track the consumption of the preservative additives and to detect and quantify up to 30 metabolites excreted by the erythrocytes. The NMR spectra of the RBC lysate provided complementary information on some biochemical pathways and set the basis for building a time-dependent red blood cell metabolic profile. DISCUSSION: We proved the analytical power of (1)H-NMR spectroscopy to study red blood cell metabolism under blood bank conditions. A potential biomarker able to provide information on the level of cellular oxidative stress protection was identified. Our data support the hypothesis that a more detailed knowledge of metabolic modifications during storage opens the way to the development of new and more effective protocols for red blood cell conservation and patient-oriented transfusion therapy.
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