Evidence map›Paper›PMID 24960545›Full record

ArticleThe Journal of clinical endocrinology and metabolism2014

Dysregulated sphingolipid metabolism in endometriosis.

Yie Hou Lee, Chin Wen Tan, Abhishek Venkatratnam, Chuen Seng Tan, Liang Cui, Seong Feei Loh, Linda Griffith, Steven R Tannenbaum, Jerry Kok Yen Chan

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of clinical endocrinology and metabolism, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 3 pooled it
6.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 3 syntheses or guidelines pooled it, 82 citations in OpenAlex.

  1. Combination of the non-invasive tests for the diagnosis of endometriosis.The Cochrane database of systematic reviews · 2016
    Pooled it
  2. Blood biomarkers for the non-invasive diagnosis of endometriosis.The Cochrane database of systematic reviews · 2016
    Pooled it
  3. Endometrial biomarkers for the non-invasive diagnosis of endometriosis.The Cochrane database of systematic reviews · 2016
    Pooled it
  4. Article
  5. Lipidomic profiling of endometrial cancer using desorption electrospray ionization mass spectrometry imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
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  8. Article
  9. Review
  10. Article
  11. Metabolomics for the identification of biomarkers in endometriosis.Archives of gynecology and obstetrics · 2024
    Review
  12. Article
  13. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Yie Hou LeeBioSym (Y.H.L., C.W.T., A.V., L.G., S.R.T.) and Infectious Diseases Inter-Disciplinary Research Groups (Y.H.L., L.C., S.R.T.), Singapore-MIT Alliance for Research and Technology, Singapore 138602; Saw Swee Hock School of Public Health (C.S.T.), National University of Singapore, Singapore 117597; Department of Reproductive Medicine (S.F.L., J.K.Y.C.), KK Women's and Children's Hospital, Singapore 229899; Departments of Biological Engineering (L.G.) and Chemistry (S.R.T.) and Center for Gynepathology Research (L.G.), Massachusetts Institute of Technology, Boston, Massachusetts 02139; Department of Obstetrics and Gynecology (J.K.Y.C.), Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228; and Cancer and Stem Cell Biology Program (J.K.Y.C.), Duke-NUS Medical School, Singapore 169857.
Chin Wen Tan
Abhishek Venkatratnam
Chuen Seng Tan
Liang Cui
Seong Feei Loh
Linda Griffith
Steven R Tannenbaum
Jerry Kok Yen Chan
Singapore-MIT Alliance for Research and Technology · SGKK Women's and Children's Hospital · SGMassachusetts Institute of Technology · USNational University Health System · SGNational University of Singapore · SG

Funding

TOXICOLOGY CORE UNITP30ES002109 · NIEHS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI LEVINE, STUART S · 1985 to 2020
$25.6M
6 · The paper itself

Abstract

backgroundIn endometriosis, the establishment and subsistence of ectopic lesions outside the endometrium suggest an altered cellular state for pathological hyperplasia. Sphingolipids are bioactive compounds, and their biosynthesis and metabolism modulate a range of cellular processes including proliferation, migration and apoptosis. We demonstrate that aberrations in sphingolipid metabolism occur in women with endometriosis.

methodsTargeted mass spectrometry on >120 sphingolipids were measured in the sera (n = 62), peritoneal fluid (n = 63), and endometrial tissue (n = 14) of women with and without endometriosis. Quantitative RT-PCR and immunohistochemistry were performed on endometrial tissues determine the expression levels of sphingolipid enzymes.

resultsSphingolipidomics identified the in vivo accumulation of numerous sphingolipids, including the functionally antagonistic glucosylceramides and ceramides in the serum and PF of women with endometriosis. We found upregulation of specific sphingolipid enzymes, namely sphingomyelin synthase 1 (SMS1), sphingomyelinase 3 (SMPD3), and glucosylceramide synthase (GCS) in the endometrium of endometriotic women with corresponding increased GlcCer, decreased sphingomyelin levels, and decreased apoptosis in the endometrium.

conclusionsOur sphingolipidomics approach provided evidence of altered sphingolipid metabolism flux in serum, peritoneal fluid, and endometrial tissue in women with endometriosis. The results provide new information on how sphingolipids and eutopic endometrium may contribute to the pathophysiology of endometriosis. The results also have implications for the use of sphingolipids as potential biomarkers.

Indexed as

AdultApoptosisAscitic FluidCell MovementCell ProliferationEndometriosisFemaleGlucosyltransferasesHumansInfertility, FemaleMembrane ProteinsMiddle AgedNerve Tissue ProteinsPeritoneal DiseasesSphingolipidsSphingomyelin Phosphodiesteraseceramide glucosyltransferaseGlucosyltransferasesMembrane ProteinsNerve Tissue ProteinsSGMS1 protein, humanSMPD3 protein, humanSphingolipidsSphingomyelin PhosphodiesteraseTransferases (Other Substituted Phosphate Groups)

Identifiers

PMID24960545
PMCPMC5393497
OpenAlexW2123076237

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.