ArticlePloS one2014
Role of TLR5 and flagella in bacillus intraocular infection.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 31 citations in OpenAlex.
- Roles of CCL2 and CCL3 in intraocular inflammation during Bacillus endophthalmitis.Experimental eye research · 2022Article
- miR-155-regulated mTOR and Toll-like receptor 5 in gastric diffuse large B-cell lymphoma.Cancer medicine · 2022Article
- Intestinal Flora: A Potential Mechanism by WhichEvidence-based complementary and alternative medicine : eCAM · 2022Article
- C-X-C Chemokines Influence Intraocular Inflammation During Bacillus Endophthalmitis.Investigative ophthalmology & visual science · 2021Article
- Immune Inhibitor A Metalloproteases Contribute to Virulence inInfection and immunity · 2021Article
- Article
- TheMicrobiology (Reading, England) · 2021Article
- Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis.Journal of visualized experiments : JoVE · 2021Article
- Innate Immune Interference Attenuates Inflammation In Bacillus Endophthalmitis.Investigative ophthalmology & visual science · 2020Article
- Multidrug-ResistantMicroorganisms · 2020Article
- Expression ofMicroorganisms · 2020Article
- The cereus matter of Bacillus endophthalmitis.Experimental eye research · 2020Review
- Article
- Targets of immunomodulation in bacterial endophthalmitis.Progress in retinal and eye research · 2019Review
- S-layer Impacts the Virulence of Bacillus in Endophthalmitis.Investigative ophthalmology & visual science · 2019Article
- Toll-Like Receptors and Relevant Emerging Therapeutics with Reference to Delivery Methods.Pharmaceutics · 2019Review
- Recent clinical trends in Toll-like receptor targeting therapeutics.Medicinal research reviews · 2019Review
- Recent Updates on Treatment of Ocular Microbial Infections by Stem Cell Therapy: A Review.International journal of molecular sciences · 2018Review
- The role of pili in Bacillus cereus intraocular infection.Experimental eye research · 2017Article
- CXCL1, but not IL-6, significantly impacts intraocular inflammation during infection.Journal of leukocyte biology · 2016Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
B. cereus possesses flagella which allow the organism to migrate within the eye during a blinding form of intraocular infection called endophthalmitis. Because flagella is a ligand for Toll-like receptor 5 (TLR5), we hypothesized that TLR5 contributed to endophthalmitis pathogenesis. Endophthalmitis was induced in C57BL/6J and TLR5-/- mice by injecting 100 CFU of B. cereus into the mid-vitreous. Eyes were analyzed for intraocular bacterial growth, retinal function, and inflammation by published methods. Purified B. cereus flagellin was also injected into the mid-vitreous of wild type C57BL/6J mice and inflammation was analyzed. TLR5 activation by B. cereus flagellin was also analyzed in vitro. B. cereus grew rapidly and at similar rates in infected eyes of C57BL/6J and TLR5-/- mice. A significant loss in retinal function in both groups of mice was observed at 8 and 12 hours postinfection. Retinal architecture disruption and acute inflammation (neutrophil infiltration and proinflammatory cytokine concentrations) increased and were significant at 8 and 12 hours postinfection. Acute inflammation was comparable in TLR5-/- and C57BL/6J mice. Physiological concentrations of purified B. cereus flagellin caused significant inflammation in C57BL/6J mouse eyes, but not to the extent of that observed during active infection. Purified B. cereus flagellin was a weak agonist for TLR5 in vitro. These results demonstrated that the absence of TLR5 did not have a significant effect on the evolution of B. cereus endophthalmitis. This disparity may be due to sequence differences in important TLR5 binding domains in B. cereus flagellin or the lack of flagellin monomers in the eye to activate TLR5 during infection. Taken together, these results suggest a limited role for flagellin/TLR5 interactions in B. cereus endophthalmitis. Based on this and previous data, the importance of flagella in this disease lies in its contribution to the motility of the organism within the eye during infection.
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