ArticleVision research2014
Ex vivo ERG analysis of photoreceptors using an in vivo ERG system.
Article in Vision research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Beyond Neovascularization: Light Response Deficits in Oxygen-Induced Retinopathy Models.Investigative ophthalmology & visual science · 2026Review
- The effects of bicarbonate on the aberrant photon response in murine rod photoreceptors.Biophysical journal · 2026Article
- Chromophore-loaded CRALBP mutant proteins restore rod function in chromophore-deficient mice.Molecular therapy. Advances · 2026Article
- Carbon Dioxide Euthanasia Selectively Affects Physiology of Murine Retinal Cells, Implicating Carbonic Anhydrase-Expressing Cell Populations.Investigative ophthalmology & visual science · 2026Article
- The physiology of dark adaptation: Progress and future directions.Progress in retinal and eye research · 2025Review
- Photoreceptor degeneration induces homeostatic rewiring of rod bipolar cells.Current biology : CB · 2025Article
- Characterization of zebrafish rod and cone photoresponses.Scientific reports · 2025Article
- Analysis of the relationship between rod cell membrane currents and the photoreceptor component of electroretinograms using a cable model.Scientific reports · 2025Article
- Long-Term Impairment of Retinal Ganglion Cell Function After Oxygen-Induced Retinopathy.Cells · 2025Article
- Protocol to perform ex vivo electroretinography on adult zebrafish.STAR protocols · 2025Article
- The USH3A causative gene clarin1 functions in Müller glia to maintain retinal photoreceptors.PLoS genetics · 2025Article
- A combination treatment based on drug repurposing demonstrates mutation-agnostic efficacy in pre-clinical retinopathy models.Nature communications · 2024Article
- The USH3A causative gene clarin1 functions in Müller glia to maintain retinal photoreceptors.bioRxiv : the preprint server for biology · 2024Article
- Full-field electroretinogram recorded with skin electrodes in 6- to 12-year-old children.Documenta ophthalmologica. Advances in ophthalmology · 2023Article
- Light drives the developmental progression of outer retinal function.The Journal of general physiology · 2023Article
- Prenylation is essential for the enrichment of cone phosphodiesterase-6 (PDE6) in outer segments and efficient cone phototransduction.Human molecular genetics · 2023Article
- An Ex Vivo Electroretinographic Apparatus for the mL-Scale Testing of Drugs to One Day and Beyond.International journal of molecular sciences · 2023Article
- The origins of the full-field flash electroretinogram b-wave.Frontiers in molecular neuroscience · 2023Review
- Bicarbonate boosts flash response amplitude to augment absolute sensitivity and extend dynamic range in murine retinal rods.Frontiers in molecular neuroscience · 2023Article
- Deletion of Protein Phosphatase 2A Accelerates Retinal Degeneration in GRK1- and Arr1-Deficient Mice.Investigative ophthalmology & visual science · 2022Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The Function of the retina and effects of drugs on it can be assessed by recording transretinal voltage across isolated retina that is perfused with physiological medium. However, building ex vivo ERG apparatus requires substantial amount of time, resources and expertise. Here we adapted a commercial in vivo ERG system for transretinal ERG recordings from rod and cone photoreceptors and compared rod and cone signaling between ex vivo and in vivo environments. We found that the rod and cone a- and b-waves recorded with the transretinal ERG adapter and a standard in vivo ERG system are comparable to those obtained from live anesthetized animals. However, ex vivo responses are somewhat slower and their oscillatory potentials are suppressed as compared to those recorded in vivo. We found that rod amplification constant (A) was comparable between ex vivo and in vivo conditions, ∼10-30s(-2) depending on the choice of response normalization. We estimate that the A in cones is between 3 and 6s(-2) in ex vivo conditions and by assuming equal A in vivo we arrive to light funnelling factor of 3 for cones in the mouse retina. The ex vivo ERG adapter provides a simple and affordable alternative to designing a custom-built transretinal recordings setup for the study of photoreceptors. Our results provide a roadmap to the rigorous quantitative analysis of rod and cone responses made possible with such a system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.