ArticleBiomicrofluidics2014
Insulator-based dielectrophoresis of mitochondria.
Article in Biomicrofluidics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Electrical Phenotyping of Aged Human Mesenchymal Stem Cells Using Dielectrophoresis.Micromachines · 2025Article
- Numerical modeling reveals improved organelle separation for dielectrophoretic ratchet migration.Electrophoresis · 2023Article
- Continuous organelle separation in an insulator-based dielectrophoretic device.Electrophoresis · 2022Article
- Particle trapping in electrically driven insulator-based microfluidics: Dielectrophoresis and induced-charge electrokinetics.Electrophoresis · 2021Review
- Microparticle transport along a planar electrode array using moving dielectrophoresis.Journal of applied physics · 2021Article
- Microscale nonlinear electrokinetics for the analysis of cellular materials in clinical applications: a review.Mikrochimica acta · 2021Review
- Isolation and identification of Listeria monocytogenes utilizing DC insulator-based dielectrophoresis.Analytica chimica acta · 2019Article
- A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors.Scientific reports · 2018Article
- Deterministic Ratchet for Sub-micrometer (Bio)particle Separation.Analytical chemistry · 2018Article
- Assessment of Sub-Micron Particles by Exploiting Charge Differences with Dielectrophoresis.Micromachines · 2017Article
- Refinement of insulator-based dielectrophoresis.The Analyst · 2017Article
- Deterministic Absolute Negative Mobility for Micro- and Submicrometer Particles Induced in a Microfluidic Device.Analytical chemistry · 2016Article
- Development of three-dimensional integrated microchannel-electrode system to understand the particles' movement with electrokinetics.Biomicrofluidics · 2016Article
- Nanohole Array-Directed Trapping of Mammalian Mitochondria Enabling Single Organelle Analysis.Analytical chemistry · 2015Article
- Isolation and enrichment of low abundant particles with insulator-based dielectrophoresis.Biomicrofluidics · 2015Article
- Microfluidic sorting of protein nanocrystals by size for X-ray free-electron laser diffraction.Structural dynamics (Melville, N.Y.) · 2015Article
- Spatial concentration distribution analysis of cells in electrode-multilayered microchannel by dielectric property measurement.Biomicrofluidics · 2015Article
- High throughput protein nanocrystal fractionation in a microfluidic sorter.Analytical chemistry · 2015Article
- Protein dielectrophoresis and the link to dielectric properties.Bioanalysis · 2015Review
- Three dimensional passivated-electrode insulator-based dielectrophoresis.Biomicrofluidics · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Isolated mitochondria display a wide range of sizes plausibly resulting from the coexistence of subpopulations, some of which may be associated with disease or aging. Strategies to separate subpopulations are needed to study the importance of these organelles in cellular functions. Here, insulator-based dielectrophoresis (iDEP) was exploited to provide a new dimension of organelle separation. The dielectrophoretic properties of isolated Fischer 344 (F344) rat semimembranosus muscle mitochondria and C57BL/6 mouse hepatic mitochondria in low conductivity buffer (0.025-0.030 S/m) at physiological pH (7.2-7.4) were studied using polydimethylsiloxane (PDMS) microfluidic devices. First, direct current (DC) and alternating current (AC) of 0-50 kHz with potentials of 0-3000 V applied over a channel length of 1 cm were separately employed to generate inhomogeneous electric fields and establish that mitochondria exhibit negative DEP (nDEP). DEP trapping potential thresholds at 0-50 kHz were also determined to be weakly dependent on applied frequency and were generally above 200 V. Second, we demonstrated a separation scheme using DC potentials <100 V to perform the first size-based iDEP sorting of mitochondria. Samples of isolated mitochondria with heterogeneous sizes (150 nm-2 μm diameters) were successfully separated into sub-micron fractions, indicating the ability to isolate mitochondria into populations based on their size.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.