ArticleThe open ophthalmology journal2014
In vitro growth of lens epithelial cells from cataract patients - association with possible risk factors for posterior capsule opacification.
Article in The open ophthalmology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- Pooled it
- Rapid posterior capsular opacification in two patients treated for negative dysphotopsias.BMC ophthalmology · 2024Article
- Optimizing Mouse Primary Lens Epithelial Cell Culture: A Comprehensive Guide to Trypsinization.Journal of visualized experiments : JoVE · 2024Article
- Five-Year Incidence of Nd:YAG Capsulotomy After Cataract Surgery with Different Single-Piece Monofocal Intraocular Lenses: A Follow-Up Study of 3955 Eyes.Clinical ophthalmology (Auckland, N.Z.) · 2024Article
- Early-Onset Posterior Capsule Opacification: Incidence, Severity, and Risk Factors.Ophthalmology and therapy · 2022Article
- Methodologies to unlock the molecular expression and cellular structure of ocular lens epithelial cells.Frontiers in cell and developmental biology · 2022Article
- Relationship between SIRT1 gene expression level and disease in age-related cataract casesTurkish journal of medical sciences · 2019Article
- Roles of TGFβ and FGF signals during growth and differentiation of mouse lens epithelial cell in vitro.Scientific reports · 2017Article
- Anterior lens capsule and epithelium thickness measurements using spectral-domain optical coherence tomography.BMC ophthalmology · 2017Observational
- Effects of lentiviral RNA interference-mediated downregulation of integrin-linked kinase on biological behaviors of human lens epithelial cells.International journal of ophthalmology · 2016Article
- Five-year incidence of Nd:YAG laser capsulotomy and association with in vitro proliferation of lens epithelial cells from individual specimens: a case control study.BMC ophthalmology · 2014Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimInter-individual differences in intrinsic proliferative capacity of lens epithelial cells may have importance for the risk of developing posterior capsule opacification (PCO) after cataract surgery. The purpose of the present study was to determine growth of human lens epithelial cells (HLEC) in culture and investigate possible associations with clinical characteristics of the donors, such as age, sex, pseudoexfoliation, uveitis and diabetes.
methodsPieces of lens capsule and adhering lens epithelial cells were obtained through capsulorhexis at cataract surgery. Specimens were cultured in a humidified CO2-incubator using standard culture medium and 5% fetal calf serum for two weeks after which cultured cells were stained with carboxy-fluorescein diacetate succinimidyl ester. Image processing software was used to determine the area of the confluent epithelial cell layer in relation to the size of the original capsule specimen.
resultsThe increase in area of confluent HLEC showed a negative correlation with diabetes at the first week after surgery. Lower age and female sex showed border-line significant associations with a higher rate of cell proliferation. The presence of pseudoexfoliation in vivo did not significantly affect cell growth in culture postoperatively. Nor did installation of xylocain in the anterior chamber during surgery.
conclusionDiabetes is associated with lower rate of proliferation of lens epithelial cells in culture. The lack of strong correlations between in vitro growth and known risk factors for PCO in the donors suggest that other factors than the proliferative capacity of the cells per se are important for PCO formation.
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