ArticleJournal of assisted reproduction and genetics2014
In vitro culture and characterization of spermatogonial stem cells on Sertoli cell feeder layer in goat (Capra hircus).
Article in Journal of assisted reproduction and genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Long-term culture and significant expansion of human Sertoli cells whilst maintaining stable global phenotype and AKT and SMAD1/5 activation.Cell communication and signaling : CCS · 2015Trial
- Advancements in Stem Cell Applications for Livestock Research: A Review.Veterinary sciences · 2025Review
- Cryopreservation and culture strategies for testicular tissue and cells in small and large animals.Frontiers in veterinary science · 2025Review
- Transcriptome Analysis Reveals Spermatogenesis-Related CircRNAs and LncRNAs in Goat Spermatozoa.Biochemical genetics · 2024Article
- Recent Progress of In Vitro 3D Culture of Male Germ Stem Cells.Journal of functional biomaterials · 2023Review
- Effects of Growth Factors on In Vitro Culture of Neonatal Piglet Testicular Tissue Fragments.Cells · 2023Article
- Perspectives: Approaches for Studying Livestock Spermatogonia.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Co-culturing or conditioned medium of Sertoli cells: Which one supports in vitro maturation of bovine oocytes and developmental competency of resulting embryos?Veterinary medicine and science · 2022Article
- A Role for Exchange of Extracellular Vesicles in Porcine Spermatogonial Co-Culture.International journal of molecular sciences · 2022Article
- Genomic Identification, Evolution, and Expression Analysis of Bromodomain Genes Family in Buffalo.Genes · 2022Article
- Culture of Kenyan Goat (Frontiers in veterinary science · 2022Article
- Culture of spermatogonial stem cells and use of surrogate sires as a breeding technology to propagate superior genetics in livestock production: A systematic review.Veterinary world · 2021Article
- Current scenario and challenges ahead in application of spermatogonial stem cell technology in livestock.Journal of assisted reproduction and genetics · 2021Review
- Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA.Journal of clinical medicine · 2021Review
- Spermatogonial Stem Cell Transplantation in Large Animals.Animals : an open access journal from MDPI · 2021Review
- Metabolic Requirements for Spermatogonial Stem Cell Establishment and Maintenance In Vivo and In Vitro.International journal of molecular sciences · 2021Review
- The role of Rho-associated kinase inhibitor, Y-27632 on primary culture of ovine spermatogonial stem cells.Animal reproduction · 2021Article
- EGF, GDNF, and IGF-1 influence the proliferation and stemness of ovine spermatogonial stem cells in vitro.Journal of assisted reproduction and genetics · 2020Article
- Manipulation of spermatogonial stem cells in livestock species.Journal of animal science and biotechnology · 2019Review
- Intratesticular injection followed by electroporation allows gene transfer in caprine spermatogenic cells.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo develop an efficient protocol for isolation, purification and long-term culture of spermatogonial stem cell (SSC) in goat.
methodsThe isolation of SSC was performed by testicular disaggregation by enzymatic digestion using collagenase IV, trypsin and DNase I. Further SSCs were enriched using Percoll density gradient centrifugation. The purity of SSCs was assessed by immunocytochemistry (ICC) using α6 integrin. The SSCs were co-cultured on Sertoli cell feeder layer. The SSC colonies were characterized by studying the expression of SSC specific markers (viz., α6 integrin and PLZF) using ICC. The abundance of mRNAs encoding the markers of SSC (viz., β1 integrin and Oct-4) and Sertoli cells (viz., vimentin) was also assayed using quantitative real-time PCR (qPCR).
resultsThe viability of isolated testicular cells was > 90 % and the Percoll density gradient method resulted in 3.65 folds enrichment with a purity of 82.5 %. Co-culturing of SSCs with Sertoli cell feeder layer allowed the maintenance of stable SSC colonies even after one and half months of culture. The results of ICC analysis showed the expression of α6 integrin and PLZF in almost all the SSC colonies. qPCR analysis revealed a differential expression of mRNAs encoding β1 integrin, Oct-4 and vimentin markers.
conclusionResults of this study demonstrate a simple enzymatic digestion and Percoll density gradient method for isolation and enrichment of SSCs, and suitability of Sertoli cell feeder layer for long term in vitro culture of SSC in goats. Results also suggest the possible application of non-caprine antibodies against SSC specific markers for the identification and subsequent assessment of SSCs in goats.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.