ArticleMetabolites2013
2-Hydrazinoquinoline as a Derivatization Agent for LC-MS-Based Metabolomic Investigation of Diabetic Ketoacidosis.
Article in Metabolites, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed.
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- Associations between hyperketonemia and the rumen metagenome and metabolome in Holstein cows during the first 2 weeks postpartum.Animal microbiome · 2025Article
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- Effect of second iron injection on growth performance, hematological parameters, and fecal microbiome of piglets fed different dietary iron levels.Journal of animal science · 2025Article
- Quantification of Total Ketone Bodies in Biological Matrices Using Stable Isotope-Labeled Internal Standards and RP-UHPLC-MS/MS.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Characterization of UrinaryMetabolites · 2024Article
- Microbiota metabolism of intestinal amino acids impacts host nutrient homeostasis and physiology.Cell host & microbe · 2024Article
- The gut microbiome regulates the clinical efficacy of sulfasalazine therapy for IBD-associated spondyloarthritis.Cell reports. Medicine · 2024Article
- Dose-Dependent Effects of Supplementing a Two-StrainAnimals : an open access journal from MDPI · 2023Article
- Specific host metabolite and gut microbiome alterations are associated with bone loss during spaceflight.Cell reports · 2023Article
- Identification of Protective Amino Acid Metabolism Events in Nursery Pigs Fed Thermally Oxidized Corn Oil.Metabolites · 2023Article
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- Regular consumption of lacto-fermented vegetables has greater effects on the gut metabolome compared with the microbiome.Gut microbiome (Cambridge, England) · 2023Article
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- Analysis of endogenous metabolites using multifunctional derivatization and capillary RPLC-MS.Analytical methods : advancing methods and applications · 2022Article
- Brief exposure of neuronal cells to levels of SCFAs observed in human systemic circulation impair lipid metabolism resulting in apoptosis.Scientific reports · 2022Article
- Identification of Independent and Shared Metabolic Responses to High-Fiber and Antibiotic Treatments in Fecal Metabolome of Grow-Finish Pigs.Metabolites · 2022Article
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- Genetic manipulation of gut microbes enables single-gene interrogation in a complex microbiome.Cell · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Short-chain carboxylic acids, aldehydes and ketones are products and regulators of many important metabolic pathways. Their levels in biofluids and tissues reflect the status of specific metabolic reactions, the homeostasis of the whole metabolic system and the wellbeing of a biological entity. In this study, the use of 2-hydrazinoquinoline (HQ) as a novel derivatization agent was explored and optimized for simultaneous liquid chromatography-mass spectrometry (LC-MS) analysis of carboxylic acids, aldehydes and ketones in biological samples. The formation of carboxylic acid derivative is attributed to the esterification reaction between HQ and a carboxyl group, while the production of aldehyde and ketone derivatives is through the formation of Schiff bases between HQ and a carbonyl group. The compatibility of HQ with biological samples was demonstrated by derivatizing urine, serum and liver extract samples. Using this HQ-based approach, the kinetics of type 1 diabetes-induced metabolic changes was characterized by the LC-MS-based metabolomic analysis of urine samples from streptozotocin (STZ)-treated mice. Subsequently, carboxylic acid, aldehyde and ketone metabolites associated with STZ-elicited disruption of nutrient and energy metabolism were conveniently identified and elucidated. Overall, HQ derivatization of carboxylic acids, aldehydes and ketones could serve as a useful tool for the LC-MS-based metabolomic investigation of endogenous metabolism.
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Registered trials
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