ArticleScientific reports2014
A minimally invasive method for retrieving single adherent cells of different types from cultures.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Contactless Single Cell Extraction From Monolayer Cell Culture Using a MEMS Acoustic Droplet Ejector.IEEE transactions on bio-medical engineering · 2025Article
- A Device for Isolation of Selected Single Adherent Cells.BioMed research international · 2022Article
- Microfluidic High-Migratory Cell Collector Suppressing Artifacts Caused by Microstructures.Micromachines · 2019Article
- Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Simultaneous Multiparameter Cellular Energy Metabolism Profiling of Small Populations of Cells.Scientific reports · 2018Article
- Platforms for Single-Cell Collection and Analysis.International journal of molecular sciences · 2018Review
- Platform for combined analysis of functional and biomolecular phenotypes of the same cell.Scientific reports · 2017Article
- Impaired coordination between signaling pathways is revealed in human colorectal cancer using single-cell mass cytometry of archival tissue blocks.Science signaling · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The field of single-cell analysis has gained a significant momentum over the last decade. Separation and isolation of individual cells is an indispensable step in almost all currently available single-cell analysis technologies. However, stress levels introduced by such manipulations remain largely unstudied. We present a method for minimally invasive retrieval of selected individual adherent cells of different types from cell cultures. The method is based on a combination of mechanical (shear flow) force and biochemical (trypsin digestion) treatment. We quantified alterations in the transcription levels of stress response genes in individual cells exposed to varying levels of shear flow and trypsinization. We report optimal temperature, RNA preservation reagents, shear force and trypsinization conditions necessary to minimize changes in the stress-related gene expression levels. The method and experimental findings are broadly applicable and can be used by a broad research community working in the field of single cell analysis.
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Registered trials
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