Evidence map›Paper›PMID 24957914›Full record

ArticleKnee surgery, sports traumatology, arthroscopy : official journal of the ESSKA2015

In vitro functional response of human tendon cells to different dosages of low-frequency pulsed electromagnetic field.

L de Girolamo, M Viganò, E Galliera, D Stanco, S Setti, M G Marazzi, G Thiebat, M M Corsi Romanelli, V Sansone

Registry-linked trialAbstract read
PubMed Publisher
In one paragraph

Article in Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05922618 (PEMFS in Patients With CRPS-I of the Foot and Ankle), which is not on this map. Cited by 23 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 2 pooled it
2.3field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05922618 nacompletednot on this mapstarted 2023, after this paper: background citation

PEMFS in Patients With CRPS-I of the Foot and Ankle: a Randomized Controlled Trial

TypeinterventionalSponsorAzienda Ospedaliero-Universitaria Consorziale Policlinico di BariRan2023 to 2025Enrolled28ConditionsPain, Joint, Complex Regional Pain Syndromes, Ankle Disease, Foot DiseasesArmsI-One
3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 43 citations in OpenAlex.

  1. Pooled it
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  5. [Effects of Electromagnetic Pulses on Exosomes Secretion by A549 Cells].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2024
    Article
  6. Viable tendon neotissue from adult adipose-derived multipotent stromal cells.Frontiers in bioengineering and biotechnology · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

L de GirolamoOrthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy. laura.degirolamo@grupposandonato.it.
M ViganòOrthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy.
E GallieraDipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, Università degli Studi di Milano, Milan, Italy.
D StancoOrthopaedic Biotechnology Laboratory, IRCCS Istituto Ortopedico Galeazzi, Via R. Galeazzi, 4, 20161, Milan, Italy.
S SettiIGEA SpA, Clinical Biophysics, Carpi, Italy.
M G MarazziDipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy.
G ThiebatSport Traumatology Unit, IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
M M Corsi RomanelliDipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy.
V SansoneDipartimento di Scienze Biomediche per la Salute, Università degli Studi di Milano, Milan, Italy.
Istituto Ortopedico Galeazzi · ITUniversity of Milan · ITIGEA Clinical Biophysics (Italy) · ITIstituti di Ricovero e Cura a Carattere Scientifico · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeChronic tendinopathy is a degenerative process causing pain and disability. Current treatments include biophysical therapies, such as pulsed electromagnetic fields (PEMF). The aim of this study was to compare, for the first time, the functional in vitro response of human tendon cells to different dosages of PEMF, varying in field intensity and duration and number of exposures.

methodsTendon cells, isolated from human semitendinosus and gracilis tendons (hTCs; n = 6), were exposed to different PEMF treatments (1.5 or 3 mT for 8 or 12 h, single or repeated treatments). Scleraxis (SCX), COL1A1, COL3A1 and vascular endothelial growth factor-A (VEGF-A) expression and cytokine production were assessed.

resultsNone of the different dosages provoked apoptotic events. Proliferation of hTCs was enhanced by all treatments, whereas only 3 mT-PEMF treatment increased cell viability. However, the single 1.5 mT-PEMF treatment elicited the highest up-regulation of SCX, VEGF-A and COL1A1 expression, and it significantly reduced COL3A1 expression with respect to untreated cells. The treated hTCs showed a significantly higher release of IL-1β, IL-6, IL-10 and TGF-β. Interestingly, the repeated 1.5 mT-PEMF significantly further increased IL-10 production.

conclusions1.5 mT-PEMF treatment was able to give the best results in in vitro healthy human tendon cell culture. Although the clinical relevance is not direct, this investigation should be considered an attempt to clarify the effect of different PEMF protocols on tendon cells, in particular focusing on the potential applicability of this cell source for regenerative medicine purpose, both in surgical and in conservative treatment for tendon disorders.

Indexed as

Electromagnetic FieldsAdultBasic Helix-Loop-Helix ProteinsCells, CulturedCell SurvivalCollagen Type ICollagen Type I, alpha 1 ChainCollagen Type IIIHumansInterleukinsTendonsTransforming Growth Factor betaUp-RegulationVascular Endothelial Growth Factor ABasic Helix-Loop-Helix ProteinsCOL3A1 protein, humanCollagen Type ICollagen Type I, alpha 1 ChainCollagen Type IIIInterleukinsSCX protein, humanTransforming Growth Factor betaVascular Endothelial Growth Factor AVEGFA protein, humanAnti-inflammatory actionHuman tendon cellsPEMF dosagePulsed electromagnetic fieldTendon markers

Identifiers

PMID24957914
OpenAlexW1977547435

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.