ArticleMetabolites2013
Gas-Chromatography Mass-Spectrometry (GC-MS) Based Metabolite Profiling Reveals Mannitol as a Major Storage Carbohydrate in the Coccolithophorid Alga Emiliania huxleyi.
Article in Metabolites, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 48 citations in OpenAlex.
- Dynamic assembly of malate dehydrogenase-citrate synthase multienzyme complex in the mitochondria.eLife · 2026Article
- Marine Algae Polysaccharides: An Overview of Characterization Techniques for Structural and Molecular Elucidation.Marine drugs · 2025Review
- Recurrent evolutionary switches of mitochondrial cytochrome c maturation systems in Archaeplastida.Nature communications · 2024Article
- Diversity of Cellulolytic Microorganisms Associated with the Subterranean TermiteJournal of fungi (Basel, Switzerland) · 2023Article
- Article
- Linear Six-Carbon Sugar Alcohols Induce Lysis ofFrontiers in microbiology · 2022Article
- Integrated algal and oil palm biorefinery as a model system for bioenergy co-generation with bioproducts and biopharmaceuticals.Bioresources and bioprocessing · 2021Review
- Metabolomics-derived marker metabolites to characterize Phaeocystis pouchetii physiology in natural plankton communities.Scientific reports · 2020Article
- Ocean acidification has little effect on the biochemical composition of the coccolithophore Emiliania huxleyi.PloS one · 2019Article
- DExSI: a new tool for the rapid quantitation of 13C-labelled metabolites detected by GC-MS.Bioinformatics (Oxford, England) · 2018Article
- Investigating mixotrophic metabolism in the model diatomPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Article
- Chemical Derivatization of Metabolite Mass Profiling of the Recretohalophyte Aeluropus lagopoides Revealing Salt Stress Tolerance Mechanism.Marine biotechnology (New York, N.Y.) · 2017Article
- Article
- Quantitative Analysis of Carbon Flow into Photosynthetic Products Functioning as Carbon Storage in the Marine Coccolithophore, Emiliania huxleyi.Marine biotechnology (New York, N.Y.) · 2015Article
- A Mitochondrially Related Plastidial Transporter Regulates Photosynthesis in the Diatom Phaeodactylum tricornutum.Physiologia plantarumArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Algae are divergent organisms having a wide variety of evolutional histories. Although most of them share photosynthetic activity, their pathways of primary carbon metabolism are rather diverse among species. Here we developed a method for gas chromatography-mass spectroscopy (GC-MS) based metabolite profiling for the coccolithophorid alga Emiliania huxleyi, which is one of the most abundant microalgae in the ocean, in order to gain an overview of the pathway of primary metabolism within this alga. Following method optimization, twenty-six metabolites could be detected by this method. Whilst most proteogenic amino acids were detected, no peaks corresponding to malate and fumarate were found. The metabolite profile of E. huxleyi was, however, characterized by a prominent accumulation of mannitol reaching in excess of 14 nmol 106 cells-1. Similarly, the accumulation of the 13C label during short term H13CO3- feeding revealed a massive redistribution of label into mannitol as well as rapid but saturating label accumulation into glucose and several amino acids including aspartate, glycine and serine. These results provide support to previous work suggesting that this species adopts C3 photosynthesis and that mannitol functions as a carbon store in E. huxleyi.
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