ArticleMetabolites2013
Metabolic Changes in Synechocystis PCC6803 upon Nitrogen-Starvation: Excess NADPH Sustains Polyhydroxybutyrate Accumulation.
Article in Metabolites, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Assessment of Polyhydroxybutyrate Production by Cyanobacteria Strains Isolated from Environmental Water Sources Using a Secondary Effluent.ACS ES&T water · 2025Article
- Controlled Carbon Loss: Threshold-Dependent Overflow Metabolism inMicroorganisms · 2025Article
- Optimising environmental factors for maximal lactate productivity in Synechocystis sp. PCC 6803 through a design of experiments approach.Biotechnology for biofuels and bioproducts · 2025Article
- Article
- Metabolic pathways to sustainability: review of purple non-sulfur bacteria potential in agri-food waste valorization.Frontiers in bioengineering and biotechnology · 2025Review
- Improving productivity of citramalate from COBiotechnology for biofuels and bioproducts · 2024Article
- A Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation inLife (Basel, Switzerland) · 2024Article
- Deciphering the genetic landscape of enhanced poly-3-hydroxybutyrate production in Synechocystis sp. B12.Biotechnology for biofuels and bioproducts · 2024Article
- Pyruvate kinase 2 from Synechocystis sp. PCC 6803 increased substrate affinity via glucose-6-phosphate and ribose-5-phosphate for phosphoenolpyruvate consumption.Plant molecular biology · 2024Article
- Glycogen deficiency enhances carbon partitioning into glutamate for an alternative extracellular metabolic sink in cyanobacteria.Communications biology · 2024Article
- Exogenous Trehalose Improves Growth, Glycogen and Poly-3-Hydroxybutyrate (PHB) Contents in Photoautotrophically GrownBiology · 2024Article
- The adc1 knockout with proC overexpression in Synechocystis sp. PCC 6803 induces a diversion of acetyl-CoA to produce more polyhydroxybutyrate.Biotechnology for biofuels and bioproducts · 2024Article
- The primary carbon metabolism in cyanobacteria and its regulation.Frontiers in plant science · 2024Review
- Improved lipid production and component of mycosporine-like amino acids by co-overexpression of amt1 and aroB genes in Synechocystis sp. PCC6803.Scientific reports · 2023Article
- Polyhydroxybutyrate Metabolism inPolymers · 2023Review
- Co-Producing Phycocyanin and Bioplastic inBiotech (Basel (Switzerland)) · 2023Article
- Site-2 Protease Slr1821 Regulates Carbon/Nitrogen Homeostasis during Ammonium Stress Acclimation in CyanobacteriumInternational journal of molecular sciences · 2023Article
- Increased Biomass and Polyhydroxybutyrate Production byInternational journal of molecular sciences · 2023Article
- Enhanced productivity of extracellular free fatty acids by gene disruptions of acyl-ACP synthetase and S-layer protein in Synechocystis sp. PCC 6803.Biotechnology for biofuels and bioproducts · 2022Article
- Combinatorial use of environmental stresses and genetic engineering to increase ethanol titres in cyanobacteria.Biotechnology for biofuels · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polyhydroxybutyrate (PHB) is a common carbon storage polymer among heterotrophic bacteria. It is also accumulated in some photoautotrophic cyanobacteria; however, the knowledge of how PHB accumulation is regulated in this group is limited. PHB synthesis in Synechocystis sp. PCC 6803 is initiated once macronutrients like phosphorus or nitrogen are limiting. We have previously reported a mutation in the gene sll0783 that impairs PHB accumulation in this cyanobacterium upon nitrogen starvation. In this study we present data which explain the observed phenotype. We investigated differences in intracellular localization of PHB synthase, metabolism, and the NADPH pool between wild type and mutant. Localization of PHB synthase was not impaired in the sll0783 mutant; however, metabolome analysis revealed a difference in sorbitol levels, indicating a more oxidizing intracellular environment than in the wild type. We confirmed this by directly measuring the NADPH/NADP ratio and by altering the intracellular redox state of wild type and sll0783 mutant. We were able to physiologically complement the mutant phenotype of diminished PHB synthase activity by making the intracellular environment more reducing. Our data illustrate that the NADPH pool is an important factor for regulation of PHB biosynthesis and metabolism, which is also of interest for potential biotechnological applications.
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