ArticleMetabolites2012
A Guideline to Univariate Statistical Analysis for LC/MS-Based Untargeted Metabolomics-Derived Data.
Article in Metabolites, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 132 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Blinded, Randomized, Controlled Study to Examine the Bioavailability of Compounds From Different Bean Varieties in Healthy Individuals.
BIAMEX: Discovery of Biomarkers of Intake of Highly Consumed Foods in Mexico by Untargeted Metabolomics
Who cites it
132 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- A priori sample size determination and power analysis in metabolic phenotyping and integrative metabolomics: an application framework based on a systematic review of literature.Metabolomics : Official journal of the Metabolomic Society · 2026Pooled it
- Rapid evaporative ionization mass spectrometry in surgery: a systematic review.The British journal of surgery · 2025Pooled it
- A systematic review on metabolomics-based diagnostic biomarker discovery and validation in pancreatic cancer.Metabolomics : Official journal of the Metabolomic Society · 2018Pooled it
- Plasma and Urinary Metabolomic Profiles Associated with the Salt Sensitivity of Blood Pressure in the Dietary Approaches to Stop Hypertension-Sodium Trial.The Journal of nutrition · 2026Trial
- Comparison of chemometric strategies for potential exposure marker discovery and false-positive reduction in untargeted metabolomics: application to the serum analysis by LC-HRMS after intake of Vaccinium fruit supplements.Analytical and bioanalytical chemistry · 2022Trial
- Altered Caffeine Metabolism Is Associated With Recurrent Hypoglycemia in Type 2 Diabetes Mellitus: A UPLC-MS-Based Untargeted Metabolomics Study.Frontiers in endocrinology · 2022Trial
- Metabolomics Effects of Folding Correction in Retinitis Pigmentosa Rhodopsin Mutant P23A.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- A Reporting Guideline for Observational Studies in Metabolomic Epidemiology: Explanation and Elaboration of the Strobe-MetEpi Checklist.Research square · 2026Article
- Metabolomic characterization and temporal dynamics of Burkholderia cepacia for polyhydroxyalkanoates production from oleic acid.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Benchtop NMR as a clinical tool for acute respiratory illness: metabolic signatures associated with disease severity.Respiratory research · 2026Article
- Integrating Metabolomics and Machine Learning for Advanced Chemical Detection.Sensors (Basel, Switzerland) · 2026Review
- Multi-omics reveals stage-specific mechanisms of Ginseng response to protopanaxadiol-type ginsenosides stress.BMC plant biology · 2026Article
- Untargeted Metabolomics Profiling of a PFAS-Exposed Flemish Population.Metabolites · 2026Article
- Effects of Balanced Dietary Patterns and/or Integrated Exercise on Serum 1,5-Anhydroglucitol and CVD Risk Factors in Individuals with Prediabetes.Life (Basel, Switzerland) · 2026Article
- Establishing an untargeted lipidomics workflow for cellular analysis: insights into endothelial cell function in anaphylaxis.Frontiers in immunology · 2026Article
- Comprehensive LC-MS Metabolomics Data Processing with notame R/Bioconductor Package.Methods in molecular biology (Clifton, N.J.) · 2026Article
- SMART 2.0 Statistical Metabolomics Analysis: An R Tool 2.0.Analytical chemistry · 2025Article
- Characterizing the physiology of circulatory arrest in humans.Nature medicine · 2025Observational
- Best practices and tools in R and Python for statistical processing and visualization of lipidomics and metabolomics data.Nature communications · 2025Review
- Mass spectrometry-based metabolomic as a powerful tool to unravel the component and mechanism in TCM.Chinese medicine · 2025Review
72 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several metabolomic software programs provide methods for peak picking, retention time alignment and quantification of metabolite features in LC/MS-based metabolomics. Statistical analysis, however, is needed in order to discover those features significantly altered between samples. By comparing the retention time and MS/MS data of a model compound to that from the altered feature of interest in the research sample, metabolites can be then unequivocally identified. This paper reports on a comprehensive overview of a workflow for statistical analysis to rank relevant metabolite features that will be selected for further MS/MS experiments. We focus on univariate data analysis applied in parallel on all detected features. Characteristics and challenges of this analysis are discussed and illustrated using four different real LC/MS untargeted metabolomic datasets. We demonstrate the influence of considering or violating mathematical assumptions on which univariate statistical test rely, using high-dimensional LC/MS datasets. Issues in data analysis such as determination of sample size, analytical variation, assumption of normality and homocedasticity, or correction for multiple testing are discussed and illustrated in the context of our four untargeted LC/MS working examples.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.