ArticleThe EMBO journal2014
Global characterization of the oocyte-to-embryo transition in Caenorhabditis elegans uncovers a novel mRNA clearance mechanism.
Article in The EMBO journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- The SPN-4 Rbfox RNA-binding protein selects maternal mRNAs for CCR4-NOT-dependent clearance in early Caenorhabditis elegans embryos.Development (Cambridge, England) · 2026Article
- Germ granule components regulate endosomal switching during the oocyte-to-embryo transition in Caenorhabditis elegans.Communications biology · 2026Article
- Programmed mitophagy at the oocyte-to-zygote transition promotes lineage endurance.Nature cell biology · 2026Article
- Poly(U) polymerase activity promotes establishment of primordial germ cells and limits somatic PGL granules in the Caenorhabditis elegans embryo.Developmental biology · 2026Article
- The SPN-4 Rbfox RNA-binding protein selects maternal mRNAs for CCR4-NOT-dependent clearance in earlybioRxiv : the preprint server for biology · 2025Article
- Article
- Landscape and regulation of mRNA translation in the early C. elegans embryo.Cell reports · 2025Article
- Landscape and regulation of mRNA translation in the earlybioRxiv : the preprint server for biology · 2025Article
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Protein profiling of zebrafish embryos unmasks regulatory layers during early embryogenesis.Cell reports · 2024Article
- Transcriptomic Analysis of the Spatiotemporal Axis of Oogenesis and Fertilization inbioRxiv : the preprint server for biology · 2024Article
- Comparative Analysis of Maternal Gene Expression Patterns Unravels Evolutionary Signatures Across Reproductive Modes.Molecular biology and evolution · 2024Article
- A spatiotemporally resolved atlas of mRNA decay in thebioRxiv : the preprint server for biology · 2024Article
- Transcriptomic analysis of the spatiotemporal axis of oogenesis and fertilization inFrontiers in cell and developmental biology · 2024Article
- Intracellular fraction of zona pellucida protein 3 is required for the oocyte-to-embryo transition in mice.Molecular human reproduction · 2023Article
- Inheritance of associative memories and acquired cellular changes in C. elegans.Nature communications · 2023Article
- Parallels and contrasts between the cnidarian and bilaterian maternal-to-zygotic transition are revealed in Hydractinia embryos.PLoS genetics · 2023Article
- Spatial single-cell sequencing of meiosis I arrested oocytes indicates acquisition of maternal transcripts from the soma.Cell reports · 2023Article
- A Compilation of the Diverse miRNA Functions inInternational journal of molecular sciences · 2023Review
- Zinc transporters ZIPT-2.4 and ZIPT-15 are required for normal C. elegans fecundity.Journal of assisted reproduction and genetics · 2022Article
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Abstract
The oocyte-to-embryo transition (OET) is thought to be mainly driven by post-transcriptional gene regulation. However, expression of both RNAs and proteins during the OET has not been comprehensively assayed. Furthermore, specific molecular mechanisms that regulate gene expression during OET are largely unknown. Here, we quantify and analyze transcriptome-wide, expression of mRNAs and thousands of proteins in Caenorhabditis elegans oocytes, 1-cell, and 2-cell embryos. This represents a first comprehensive gene expression atlas during the OET in animals. We discovered a first wave of degradation in which thousands of mRNAs are cleared shortly after fertilization. Sequence analysis revealed a statistically highly significant presence of a polyC motif in the 3' untranslated regions of most of these degraded mRNAs. Transgenic reporter assays demonstrated that this polyC motif is required and sufficient for mRNA degradation after fertilization. We show that orthologs of human polyC-binding protein specifically bind this motif. Our data suggest a mechanism in which the polyC motif and binding partners direct degradation of maternal mRNAs. Our data also indicate that endogenous siRNAs but not miRNAs promote mRNA clearance during the OET.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.