ArticleScientific reports2014
Quantitatively mapping cellular viscosity with detailed organelle information via a designed PET fluorescent probe.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 146 citations in OpenAlex.
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- Mapping cell dynamics in human ex vivo hair follicles suggests pulling mechanism of hair growth.Nature communications · 2025Article
- Chemical propulsion of hemozoin crystal motion in malaria parasites.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Dynamic Cytoplasm: A Physical Regulator of Enzymatic Function.Biochemistry · 2025Review
- Unraveling Hydration Shell Dynamics and Viscosity Effects Around Cyanamide Probes via 2D IR Spectroscopy.Journal of the American Chemical Society · 2025Article
- Luminescence Fingerprint of Intracellular NIR-II Gold Nanocluster Transformation: Implications for Sensing and Imaging.ACS nano · 2025Article
- How and when to measure mitochondrial inner membrane potentials.Biophysical journal · 2024Review
- Phasor identifier: A cloud-based analysis of phasor-FLIM data on Python notebooks.Biophysical reports · 2023Article
- Fluorescence lifetime Hong-Ou-Mandel sensing.Nature communications · 2023Article
- Dense and Acidic Organelle-Targeted Visualization in Living Cells: Application of Viscosity-Responsive Fluorescence Utilizing Restricted Access to Minimum Energy Conical Intersection.Analytical chemistry · 2023Article
- A Novel Fluorescent Probe for Determination of pH and Viscosity Based on a Highly Water-Soluble 1,8-Naphthalimide Rotor.Molecules (Basel, Switzerland) · 2022Article
- HydroFlipper membrane tension probes: imaging membrane hydration and mechanical compression simultaneously in living cells.Chemical science · 2022Article
- A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
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- A dynamical model of oncotripsy by mechanical cell fatigue: selective cancer cell ablation by low-intensity pulsed ultrasound.Proceedings. Mathematical, physical, and engineering sciences · 2020Article
- Towards the directed evolution of protein materials.MRS communications · 2019Article
- Population analysis to increase the robustness of molecular computational identification and its extension into the near-infrared for substantial numbers of small objects.Chemical science · 2019Article
- A Threshold-Limited Fluorescence Probe for Viscosity.Frontiers in chemistry · 2019Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Viscosity is a fundamental physical parameter that influences diffusion in biological processes. The distribution of intracellular viscosity is highly heterogeneous, and it is challenging to obtain a full map of cellular viscosity with detailed organelle information. In this work, we report 1 as the first fluorescent viscosity probe which is able to quantitatively map cellular viscosity with detailed organelle information based on the PET mechanism. This probe exhibited a significant ratiometric fluorescence intensity enhancement as solvent viscosity increases. The emission intensity increase was attributed to combined effects of the inhibition of PET due to restricted conformational access (favorable for FRET, but not for PET), and the decreased PET efficiency caused by viscosity-dependent twisted intramolecular charge transfer (TICT). A full map of subcellular viscosity was successfully constructed via fluorescent ratiometric detection and fluorescence lifetime imaging; it was found that lysosomal regions in a cell possess the highest viscosity, followed by mitochondrial regions.
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