ArticleMetabolites2014
Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris.
Article in Metabolites, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Metabolic engineering ofApplied and environmental microbiology · 2026Review
- Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid.Microbial cell factories · 2023Article
- Ammonium sulfate supplementation enhances erythromycin biosynthesis by augmenting intracellular metabolism and precursor supply in Saccharopolyspora erythraea.Bioprocess and biosystems engineering · 2023Article
- Streamlined identification of strain engineering targets for bioprocess improvement using metabolic pathway enrichment analysis.Scientific reports · 2023Article
- Second generation Pichia pastoris strain and bioprocess designs.Biotechnology for biofuels and bioproducts · 2022Review
- Metabolic Footprinting of Microbial Systems Based on Comprehensive In Silico Predictions of MS/MS Relevant Data.Metabolites · 2022Article
- Bioreactor-scale cell performance and protein production can be substantially increased by using a secretion signal that drives co-translational translocation in Pichia pastoris.New biotechnology · 2021Article
- Engineering cofactor metabolism for improved protein and glucoamylase production in Aspergillus niger.Microbial cell factories · 2020Article
- Continuous Cultivation as a Tool Toward the Rational Bioprocess Development WithFrontiers in bioengineering and biotechnology · 2020Review
- Harnessing microbial metabolomics for industrial applications.World journal of microbiology & biotechnology · 2019Review
- Elevated temperatures do not trigger a conserved metabolic network response among thermotolerant yeasts.BMC microbiology · 2019Article
- Ex vivo and in vivo stable isotope labelling of central carbon metabolism and related pathways with analysis by LC-MS/MS.Nature protocols · 2019Article
- Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol metabolism in Pichia pastoris.Scientific reports · 2017Article
- Rapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling.Journal of industrial microbiology & biotechnology · 2017Article
- CombinedBioresources and bioprocessing · 2016Article
- Systems-level organization of yeast methylotrophic lifestyle.BMC biology · 2015Article
- Integrated isotope-assisted metabolomics and (13)C metabolic flux analysis reveals metabolic flux redistribution for high glucoamylase production by Aspergillus niger.Microbial cell factories · 2015Article
- Engineering the supply chain for protein production/secretion in yeasts and mammalian cells.Journal of industrial microbiology & biotechnology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of the production of a Rhizopus oryzae lipase (Rol) on P. pastoris central carbon metabolism. Higher oxygen uptake and CO2 production rates and slightly reduced biomass yield suggest an increased energy demand for the producing strain. This observation is further confirmed by 13C-based metabolic flux analysis. In particular, the flux through the methanol oxidation pathway and the TCA cycle was increased in the Rol-producing strain compared to the reference strain. Next to changes in the flux distribution, significant variations in intracellular metabolite concentrations were observed. Most notably, the pools of trehalose, which is related to cellular stress response, and xylose, which is linked to methanol assimilation, were significantly increased in the recombinant strain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.