ArticleMetabolites2014
Stable isotope tracer analysis in isolated mitochondria from mammalian systems.
Article in Metabolites, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Targeting fatty acid oxidation enhances response to HER2-targeted therapy.Nature communications · 2024Article
- Skeletal Muscle Metabolism Is Dynamic during Porcine Postnatal Growth.Metabolites · 2024Article
- Inferring mitochondrial and cytosolic metabolism by coupling isotope tracing and deconvolution.Nature communications · 2023Article
- Isotope-Assisted Metabolic Flux Analysis: A Powerful Technique to Gain New Insights into the Human Metabolome in Health and Disease.Metabolites · 2022Review
- Sub-Cellular Metabolomics Contributes Mitochondria-Specific Metabolic Insights to a Mouse Model of Leigh Syndrome.Metabolites · 2021Article
- Cholesterol-Induced Metabolic Reprogramming in Breast Cancer Cells Is Mediated via the ERRα Pathway.Cancers · 2021Article
- UCP1 governs liver extracellular succinate and inflammatory pathogenesis.Nature metabolism · 2021Review
- Transporters at the Interface between Cytosolic and Mitochondrial Amino Acid Metabolism.Metabolites · 2021Review
- Forces, fluxes, and fuels: tracking mitochondrial metabolism by integrating measurements of membrane potential, respiration, and metabolites.American journal of physiology. Cell physiology · 2021Review
- CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice.Science translational medicine · 2020Article
- Glucose-dependent partitioning of arginine to the urea cycle protects β-cells from inflammation.Nature metabolism · 2020Article
- Review
- Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism.Molecular metabolism · 2019Article
- Studying metabolic flux adaptations in cancer through integrated experimental-computational approaches.BMC biology · 2019Review
- Spatial-fluxomics provides a subcellular-compartmentalized view of reductive glutamine metabolism in cancer cells.Nature communications · 2019Article
- High-Speed Tracer Analysis of Metabolism (HS-TrAM).Wellcome open research · 2018Article
- Struggling for breath in Sherbrooke - 1st Symposium on "One mitochondrion, many diseases" in Sherbrooke, Québec, Canada, March 11th, 2015.Microbial cell (Graz, Austria) · 2015Article
- Mitochondria, energetics, epigenetics, and cellular responses to stress.Environmental health perspectives · 2014Review
- Unraveling biochemical pathways affected by mitochondrial dysfunctions using metabolomic approaches.Metabolites · 2014Review
- Metformin directly acts on mitochondria to alter cellular bioenergetics.Cancer & metabolism · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria are a focal point in metabolism, given that they play fundamental roles in catabolic, as well as anabolic reactions. Alterations in mitochondrial functions are often studied in whole cells, and metabolomics experiments using 13C-labeled substrates, coupled with mass isotopomer distribution analyses, represent a powerful approach to study global changes in cellular metabolic activities. However, little is known regarding the assessment of metabolic activities in isolated mitochondria using this technology. Studies on isolated mitochondria permit the evaluation of whether changes in cellular metabolic activities are due to modifications in the intrinsic properties of the mitochondria. Here, we present a streamlined approach to accurately determine 13C, as well as 12C enrichments in isolated mitochondria from mammalian tissues or cultured cells by GC/MS. We demonstrate the relevance of this experimental approach by assessing the effects of drugs perturbing mitochondrial functions on the mass isotopomer enrichment of metabolic intermediates. Furthermore, we investigate 13C and 12C enrichments in mitochondria isolated from cancer cells given the emerging role of metabolic alterations in supporting tumor growth. This original method will provide a very sensitive tool to perform metabolomics studies on isolated mitochondria.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.