ArticleMetabolites2014
Application of stable isotope-assisted metabolomics for cell metabolism studies.
Article in Metabolites, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 50 citations in OpenAlex.
- Stable Isotope-Resolved Metabolomics in Elucidating Mitochondrial Metabolic Reprogramming and Therapeutic Targets.Biomedical chromatography : BMC · 2026Review
- Deciphering sphingolipid biosynthesis dynamics iniScience · 2024Article
- md_harmonize: A Python Package for Atom-Level Harmonization of Public Metabolic Databases.Metabolites · 2023Article
- Metabolomics as an emerging tool to study plant-microbe interactions.Emerging topics in life sciences · 2022Review
- Reference Standardization for Quantification and Harmonization of Large-Scale Metabolomics.Analytical chemistry · 2020Article
- A shift in glutamine nitrogen metabolism contributes to the malignant progression of cancer.Nature communications · 2020Article
- Applications of Chromatography-Ultra High-Resolution MS for Stable Isotope-Resolved Metabolomics (SIRM) Reconstruction of Metabolic Networks.Trends in analytical chemistry : TRAC · 2020Article
- Stable Isotope-Labeled Lipidomics to Unravel the Heterogeneous Development Lipotoxicity.Molecules (Basel, Switzerland) · 2018Article
- Identification of metabolism-associated genes and pathways involved in different stages of clear cell renal cell carcinoma.Oncology letters · 2018Article
- Metabolomics of Early Stage Plant Cell-Microbe Interaction Using Stable Isotope Labeling.Frontiers in plant science · 2018Article
- The Chemistry of Plant-Microbe Interactions in the Rhizosphere and the Potential for Metabolomics to Reveal Signaling Related to Defense Priming and Induced Systemic Resistance.Frontiers in plant science · 2018Review
- Flux Analysis of Free Amino Sugars and Amino Acids in Soils by Isotope Tracing with a Novel Liquid Chromatography/High Resolution Mass Spectrometry Platform.Analytical chemistry · 2017Article
- Glutamate metabolism in HIV-1 infected macrophages: Role of HIV-1 Vpr.Cell cycle (Georgetown, Tex.) · 2016Article
- Mathematical Modeling and Dynamic Simulation of Metabolic Reaction Systems Using Metabolome Time Series Data.Frontiers in molecular biosciences · 2016Review
- Cancer Metabolism and Drug Resistance.Metabolites · 2015Review
- Impact of kinetic isotope effects in isotopic studies of metabolic systems.BMC systems biology · 2015Article
- Novel strategy for non-targeted isotope-assisted metabolomics by means of metabolic turnover and multivariate analysis.Metabolites · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The applications of stable isotopes in metabolomics have facilitated the study of cell metabolisms. Stable isotope-assisted metabolomics requires: (1) properly designed tracer experiments; (2) stringent sampling and quenching protocols to minimize isotopic alternations; (3) efficient metabolite separations; (4) high resolution mass spectrometry to resolve overlapping peaks and background noises; and (5) data analysis methods and databases to decipher isotopic clusters over a broad m/z range (mass-to-charge ratio). This paper overviews mass spectrometry based techniques for precise determination of metabolites and their isotopologues. It also discusses applications of isotopic approaches to track substrate utilization, identify unknown metabolites and their chemical formulas, measure metabolite concentrations, determine putative metabolic pathways, and investigate microbial community populations and their carbon assimilation patterns. In addition, 13C-metabolite fingerprinting and metabolic models can be integrated to quantify carbon fluxes (enzyme reaction rates). The fluxome, in combination with other "omics" analyses, may give systems-level insights into regulatory mechanisms underlying gene functions. More importantly, 13C-tracer experiments significantly improve the potential of low-resolution gas chromatography-mass spectrometry (GC-MS) for broad-scope metabolism studies. We foresee the isotope-assisted metabolomics to be an indispensable tool in industrial biotechnology, environmental microbiology, and medical research.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.