ArticleJournal of functional biomaterials2013
Epoxy cross-linked collagen and collagen-laminin Peptide hydrogels as corneal substitutes.
Article in Journal of functional biomaterials, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Seeing through collagen: integrative pro-regenerative corneal implants for clearer future.NPJ Regenerative medicine · 2026Review
- Chemical Crosslinking of Acid Soluble Collagen Fibres.Biomimetics (Basel, Switzerland) · 2025Review
- Review of collagen type I-based hydrogels: focus on composition-structure-properties relationships.npj biomedical innovations · 2025Review
- Modulating pro-adhesive nature of metallic surfaces through a polypeptide coupling via diazonium chemistry.Scientific reports · 2023Article
- Controlled Drug Delivery Device for Cornea Treatment and Novel Method for Its Testing.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Improving the properties of fish skin collagen/silk fibroin dressing by chemical treatment for corneal wound healing.International wound journal · 2023Article
- A Comprehensive Review on Collagen Type I Development of Biomaterials for Tissue Engineering: From Biosynthesis to Bioscaffold.Biomedicines · 2022Review
- Crosslinker-free collagen gelation for corneal regeneration.Scientific reports · 2022Article
- Tuning gelatin-based hydrogel towards bioadhesive ocular tissue engineering applications.Bioactive materials · 2021Article
- Collagen- and hyaluronic acid-based hydrogels and their biomedical applications.Materials science & engineering. R, Reports : a review journal · 2021Article
- Optimization of Collagen Chemical Crosslinking to Restore Biocompatibility of Tissue-Engineered Scaffolds.Pharmaceutics · 2021Article
- Advanced Strategies for Tissue Engineering in Regenerative Medicine: A Biofabrication and Biopolymer Perspective.Molecules (Basel, Switzerland) · 2021Review
- Significance of Crosslinking Approaches in the Development of Next Generation Hydrogels for Corneal Tissue Engineering.Pharmaceutics · 2021Review
- Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts.Acta biomaterialia · 2019Article
- The effect of the carbodiimide cross-linker on the structural and biocompatibility properties of collagen-chondroitin sulfate electrospun mat.International journal of nanomedicine · 2018Article
- Capturing relevant extracellular matrices for investigating cell migration.F1000Research · 2015Review
- An in vitro 3-dimensional Collagen-based Corneal Construct with Innervation Using Human Corneal Cell Lines.Ophthalmology scienceArticle
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collagen were directly cross-linked using l,4-Butanediol diglycidyl ether (BDDGE) under basic conditions at pH 11. Alternatively, under conventional methodology, using both BDDGE and 1-Ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) as cross-linkers, hydrogels were fabricated under acidic conditions. In this latter strategy, Cu(BF4)2·XH2O was used to catalyze the formation of secondary amine bonds. To date, we have demonstrated that both methods of chemical cross-linking improved the elasticity and tensile strength of the collagen implants. Differential scanning calorimetry and biocompatibility studies indicate comparable, and in some cases, enhanced properties compared to that of the EDC/NHS controls. In vitro studies showed that human corneal epithelial cells and neuronal progenitor cell lines proliferated on these hydrogels. In addition, improvement of cell proliferation on the surfaces of the materials was observed when neurite promoting laminin epitope, IKVAV, and adhesion peptide, YIGSR, were incorporated. However, the elasticity decreased with peptide incorporation and will require further optimization. Nevertheless, we have shown that epoxy cross-linkers should be further explored in the fabrication of collagen-based hydrogels, as alternatives to or in conjunction with carbodiimide cross-linkers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.