Evidence map›Paper›PMID 24955895›Full record

ArticleLaboratory investigation; a journal of technical methods and pathology2014

LL-37 secreted by epithelium promotes fibroblast collagen production: a potential mechanism of small airway remodeling in chronic obstructive pulmonary disease.

Congcong Sun, Maoxiang Zhu, Zhihua Yang, Xiujie Pan, Yuke Zhang, Qin Wang, Wei Xiao

Abstract read
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Article in Laboratory investigation; a journal of technical methods and pathology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
2.6field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 49 citations in OpenAlex.

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  16. Formyl peptide receptors in the mucosal immune system.Experimental & molecular medicine · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Congcong SunDepartment of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan, China.
Maoxiang ZhuDepartment of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing, China.
Zhihua YangDepartment of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing, China.
Xiujie PanDepartment of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing, China.
Yuke ZhangDepartment of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan, China.
Qin WangDepartment of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan, China.
Wei XiaoDepartment of Respiratory Medicine, Qilu Hospital, Shandong University, Jinan, China.
Qilu Hospital of Shandong University · CNBeijing Radiation Center · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Emerging evidence suggests that the process of small airway remodeling is mediated by profibrotic growth factors produced by epithelium, which are capable of activating the underlying mesenchymal cells with excessive collagen production. It has been demonstrated that human cathelicidin antimicrobial protein LL-37 is highly expressed in small airway epithelium from COPD patients. However, it is unknown whether the increased levels of LL-37 in epithelium are involved in the pathogenesis of small airway remodeling in COPD. In this study, we examined the expression of LL-37 in small airways from smokers with COPD and controls (non-smokers and smokers without COPD) by immunohistochemistry, and then the association between LL-37 expression in epithelium and the structural changes of small airway remodeling was analyzed. In vitro, the effect of CSE-induced epithelial secretion of LL-37 on collagen production in human lung fibroblasts (HFL-1 cell line) was studied in a co-culture system. Finally, the signaling pathways involved in the effect of LL-37 on fibroblast collagen production were evaluated. The results showed that LL-37 immunoreactivity in airway epithelium was significantly elevated in smokers with COPD compared with controls. In addition, the magnitude of LL-37 expression in epithelium was positively correlated with airway wall thickness and collagen deposition. In vitro, CSE-induced epithelial secretion of LL-37 promoted fibroblast collagen production. Finally, we showed that formyl peptide receptor-like 1 (FPRL1)-dependent extracellular signal-regulated kinase (ERK) signaling pathway was essential for LL-37-induced collagen production in HFL-1 cells. These results suggest that after cigarette smoke exposure, the increased levels of LL-37 in airway epithelium could stimulate collagen production in the underlying lung fibroblasts and may contribute to small airway remodeling in COPD.

Indexed as

Airway RemodelingAdultAmino Acid SequenceAntimicrobial Cationic PeptidesBase SequenceCase-Control StudiesCathelicidinsCell LineCollagenDNA PrimersFemaleFibroblastsHumansMaleMiddle AgedMolecular Sequence DataAntimicrobial Cationic PeptidesCathelicidinsCollagenDNA Primers

Identifiers

PMID24955895
OpenAlexW1998661621

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.