ArticlePloS one2014
Human intestinal cells modulate conjugational transfer of multidrug resistance plasmids between clinical Escherichia coli isolates.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Conjugation frequency of ESBL- and pAmpC- E. coli in broiler chickens in vivo and in vitro.BMC microbiology · 2026Article
- Population Ecology-Quantitative Microbial Risk Assessment (QMRA) Model for Antibiotic-Resistant and SusceptibleEnvironmental science & technology · 2025Article
- The gut microbiome: an emerging epicenter of antimicrobial resistance?Frontiers in microbiology · 2025Review
- In situ, in vivo, and in vitro approaches for studying AMR plasmid conjugation in the gut microbiome.FEMS microbiology reviews · 2023Review
- Article
- Models for Gut-Mediated Horizontal Gene Transfer by Bacterial Plasmid Conjugation.Frontiers in microbiology · 2022Review
- Chicken gut microbiome members limit the spread of an antimicrobial resistance plasmid inProceedings. Biological sciences · 2021Article
- Transfer of Antibiotic Resistance Plasmid from CommensalLife (Basel, Switzerland) · 2021Article
- Molecular Mechanisms Influencing Bacterial Conjugation in the Intestinal Microbiota.Frontiers in microbiology · 2021Review
- Highly efficient gene transfer in the mouse gut microbiota is enabled by the InclCommunications biology · 2020Article
- The human gut resistome: Current concepts & future prospects.The Indian journal of medical research · 2019Review
- Antibiotic Treatment Drives the Diversification of the Human Gut Resistome.Genomics, proteomics & bioinformatics · 2019Article
- Prokaryotic horizontal gene transfer within the human holobiont: ecological-evolutionary inferences, implications and possibilities.Microbiome · 2018Review
- AnmBio · 2017Article
- Selection and Transmission of Antibiotic-Resistant Bacteria.Microbiology spectrum · 2017Review
- Addressing the Antibiotic Resistance Problem with Probiotics: Reducing the Risk of Its Double-Edged Sword Effect.Frontiers in microbiology · 2016Review
- The human gut resistome.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Aging and the human gut microbiota-from correlation to causality.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial conjugation in the human gut microbiota is believed to play a major role in the dissemination of antibiotic resistance genes and virulence plasmids. However, the modulation of bacterial conjugation by the human host remains poorly understood and there is a need for controlled systems to study this process. We established an in vitro co-culture system to study the interaction between human intestinal cells and bacteria. We show that the conjugation efficiency of a plasmid encoding an extended spectrum beta-lactamase is reduced when clinical isolates of Escherichia coli are co-cultured with human intestinal cells. We show that filtered media from co-cultures contain a factor that reduces conjugation efficiency. Protease treatment of the filtered media eliminates this inhibition of conjugation. This data suggests that a peptide or protein based factor is secreted on the apical side of the intestinal cells exposed to bacteria leading to a two-fold reduction in conjugation efficiency. These results show that human gut epithelial cells can modulate bacterial conjugation and may have relevance to gene exchange in the gut.
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