ArticleTheranostics2014
Exploration of panviral proteome: high-throughput cloning and functional implications in virus-host interactions.
Article in Theranostics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- NeuroViOme: a viral orfeome collection for studies of neurodegenerative disease.Journal of neurovirology · 2026Review
- Pooled PPIseq: Screening the SARS-CoV-2 and human interface with a scalable multiplexed protein-protein interaction assay platform.PloS one · 2025Article
- Gaining insights on immune responses to the novel coronavirus, COVID-19 and therapeutic challenges.Life sciences · 2020Article
- Experimental Analysis of Viral-Host Interactions.Frontiers in physiology · 2019Review
- Legionella effector AnkX interacts with host nuclear protein PLEKHN1.BMC microbiology · 2018Article
- Multiplexed Nucleic Acid Programmable Protein Arrays.Theranostics · 2017Article
- Review
- Proteomic approaches to uncovering virus-host protein interactions during the progression of viral infection.Expert review of proteomics · 2016Review
- Sustainability in a Hospital-Based Biobank and University-Based DNA Biorepository: Strategic Roadmaps.Biopreservation and biobanking · 2015Article
- Antiviral antibody profiling by high-density protein arrays.Proteomics · 2015Article
- High-throughput identification of proteins with AMPylation using self-assembled human protein (NAPPA) microarrays.Nature protocols · 2015Article
- NAPPA as a Real New Method for Protein Microarray Generation.Microarrays (Basel, Switzerland) · 2015Review
- Host-pathogen interaction profiling using self-assembling human protein arrays.Journal of proteome research · 2015Article
- Site-specific recombinational cloning using gateway and in-fusion cloning schemes.Current protocols in molecular biology · 2015Article
- AMPylation of Rho GTPases subverts multiple host signaling processes.The Journal of biological chemistry · 2014Article
- Copper-catalyzed azide-alkyne cycloaddition (click chemistry)-based detection of global pathogen-host AMPylation on self-assembled human protein microarrays.Molecular & cellular proteomics : MCP · 2014Article
- Multidisciplinary efforts driving translational theranostics.Theranostics · 2014Article
- Pediatric biobanks and parents of disabled children associations opinions on establishing children repositories in developing countries.Journal of medicine and lifeArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Throughout the long history of virus-host co-evolution, viruses have developed delicate strategies to facilitate their invasion and replication of their genome, while silencing the host immune responses through various mechanisms. The systematic characterization of viral protein-host interactions would yield invaluable information in the understanding of viral invasion/evasion, diagnosis and therapeutic treatment of a viral infection, and mechanisms of host biology. With more than 2,000 viral genomes sequenced, only a small percent of them are well investigated. The access of these viral open reading frames (ORFs) in a flexible cloning format would greatly facilitate both in vitro and in vivo virus-host interaction studies. However, the overall progress of viral ORF cloning has been slow. To facilitate viral studies, we are releasing the initiation of our panviral proteome collection of 2,035 ORF clones from 830 viral genes in the Gateway® recombinational cloning system. Here, we demonstrate several uses of our viral collection including highly efficient production of viral proteins using human cell-free expression system in vitro, global identification of host targets for rubella virus using Nucleic Acid Programmable Protein Arrays (NAPPA) containing 10,000 unique human proteins, and detection of host serological responses using micro-fluidic multiplexed immunoassays. The studies presented here begin to elucidate host-viral protein interactions with our systemic utilization of viral ORFs, high-throughput cloning, and proteomic technologies. These valuable plasmid resources will be available to the research community to enable continued viral functional studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.