ArticleInternational journal of microbiology2014
Molecular Typing of Methicillin Resistant Staphylococcus aureus Clinical Isolates on the Basis of Protein A and Coagulase Gene Polymorphisms.
Article in International journal of microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- Epidemiology of clinically isolated methicillin-resistant Staphylococcus aureus (MRSA) and its susceptibility to linezolid and vancomycin in Egypt: a systematic review with meta-analysis.BMC infectious diseases · 2023Pooled it
- From Hospital to Community: Exploring Antibiotic Resistance and Genes Associated with Virulence Factor Diversity of Coagulase-PositiveAntibiotics (Basel, Switzerland) · 2023Article
- Comparative Genotypic Analysis of RAPD and RFLP Markers for Molecular Variation Detection of Methicillin-ResistantMedicina (Kaunas, Lithuania) · 2022Article
- Mupirocin-ResistantBioMed research international · 2022Article
- Characterization of methicillin-resistant3 Biotech · 2020Article
- Molecular Characterization, Drug Resistance and Virulence Analysis of Constitutive and Inducible Clindamycin ResistanceInfection and drug resistance · 2020Article
- Prevalence, Genetic Diversity, and Temporary Shifts of Inducible Clindamycin ResistanceFrontiers in microbiology · 2020Article
- Genetic Variability of Methicillin ResistantOsong public health and research perspectives · 2019Article
- Detection of coagulase gene inVeterinary world · 2019Article
- Molecular typing of3 Biotech · 2018Article
- Detection of Panton-valentine Leukocidin Gene Isoforms ofAdvanced biomedical research · 2017Article
- Molecular typing of nosocomialIranian journal of basic medical sciences · 2016Article
- Association of Environmental Contamination in the Home With the Risk for Recurrent Community-Associated, Methicillin-Resistant Staphylococcus aureus Infection.JAMA internal medicine · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increased frequency of methicillin-resistant Staphylococcus aureus (MRSA) in hospitalized patients requires rapid and reliable characterization of isolates for control of MRSA spread in hospitals. This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A (spa) and coagulase (coa) gene polymorphisms to verify their ability in assessing the relatedness of isolates. Seventy-five MRSA isolates, from different ICUs of Alexandria University Main Hospital, were characterized using antibiotyping and PCR-RFLP analysis of coa and spa genes. Thirty-two antibiotypes were identified. coa gene PCR generated 3 types and 10 subtypes of band patterns. HaeIII restriction digestion of amplified coa gene products produced 5 major banding patterns and 12 subtypes. spa gene PCR products generated 4 major and 11 minor types, and their HaeII restriction digestion showed 5 major and 12 minor banding patterns. The combined coa and spa RFLP patterns generated 22 combined R types. Typing using coa PCR and PCR-RFLP had the same discriminatory index (DI) value (0.64), which was comparable to that of both spa PCR and PCR-RFLP techniques (0.68). The combined grouping increased the DI value to 0.836. The current study revealed that testing for multiple gene polymorphisms is more useful for local epidemiologic purposes.
Identifiers
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Registered trials
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