Evidence map›Paper›PMID 24954155›Full record

ArticleDevelopmental biology2014

Identification of a transient Sox5 expressing progenitor population in the neonatal ventral forebrain by a novel cis-regulatory element.

Hailing Hao, Ying Li, Evangeline Tzatzalos, Jordana Gilbert, Dhara Zala, Mantu Bhaumik, Li Cai

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Hailing HaoDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, United States.
Ying LiDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, United States.
Evangeline TzatzalosDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, United States.
Jordana GilbertDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, United States.
Dhara ZalaDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, United States.
Mantu BhaumikThe Child Health Institute of New Jersey, 89 French Street, New Brunswick, NJ 08901, United States.
Li CaiDepartment of Biomedical Engineering, Rutgers University, 599 Taylor Road, Piscataway, NJ 08854, United States. Electronic address: lcai@rutgers.edu.
Rutgers, The State University of New Jersey · US

Funding

TRANSCRIPTIONAL PROFILINGP30CA072720 · NCI · UNIV OF MED/DENT NJ-R W JOHNSON MED SCH · PI Chang S Chan · 1997 to 2026
$94.5M
T32 Training Program in Mechanisms and Innovation in Vascular DiseaseT32HL098049 · NHLBI · STANFORD UNIVERSITY · PI Nicholas James Leeper, Philip S Tsao · 2010 to 2026
$6.3M
Comparative genomic and molecular genetics approaches to retinal stem cellsR21EY018738 · NEI · RUTGERS, THE STATE UNIV OF N.J. · PI CAI, LI · 2008 to 2009
$412k
NCI NIH HHS P30 CA072720NCI NIH HHS P30CA072720NEI NIH HHS EY018738NEI NIH HHS R21 EY018738NHLBI NIH HHS T32 HL098049
6 · The paper itself

Abstract

Precise control of lineage-specific gene expression in the neural stem/progenitor cells is crucial for generation of the diversity of neuronal and glial cell types in the central nervous system (CNS). The mechanism underlying such gene regulation, however, is not fully elucidated. Here, we report that a 377 bp evolutionarily conserved DNA fragment (CR5), located approximately 32 kbp upstream of Olig2 transcription start site, acts as a cis-regulator for gene expression in the development of the neonatal forebrain. CR5 is active in a time-specific and brain region-restricted manner. CR5 activity is not detected in the embryonic stage, but it is exclusively in a subset of Sox5+ cells in the neonatal ventral forebrain. Furthermore, we show that Sox5 binding motif in CR5 is important for this cell-specific gene regulatory activity; mutation of Sox5 binding motif in CR5 alters reporter gene expression with different cellular composition. Together, our study provides new insights into the regulation of cell-specific gene expression during CNS development.

Indexed as

AnimalsBase SequenceBasic Helix-Loop-Helix ProteinsBinding SitesCattleEnhancer Elements, GeneticGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMiceMice, Inbred C57BLMice, TransgenicNerve Tissue ProteinsNeural Stem CellsNeurogenesisOligodendrocyte Transcription Factor 2ProsencephalonBasic Helix-Loop-Helix ProteinsGreen Fluorescent ProteinsNerve Tissue ProteinsOlig2 protein, mouseOligodendrocyte Transcription Factor 2Sox5 protein, mouseSOXD Transcription FactorsCis-elementGene regulationNeural stem/progenitor cellsOlig2Sox5Trans-acting factor

Identifiers

PMID24954155
PMCPMC4167819
OpenAlexW1980184748

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.