ArticleCurrent biology : CB2014
Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.
Article in Current biology : CB, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 177 papers.
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Who cites it
177 citing papers in PubMed, 300 citations in OpenAlex.
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- The CENP-A chaperone complex spatially organizes centromeres.Science advances · 2026Article
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- Electrospray Regime Controls Sensitivity and Quantitative Performance in Micro- and Nano-Flow CE-ESI-MS.Analytical chemistry · 2026Article
- Histone Density and Dynamics Shape Mitotic Chromatid Architecture in Xenopus Egg Extracts.Genes to cells : devoted to molecular & cellular mechanisms · 2026Article
- Human mitotic spindles as active liquid crystals: From collective behaviors to discrete filaments.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- In-Cell Proteomics Enables High-Resolution Spatial and Temporal Mapping of Early Xenopus tropicalis Embryos.Molecular & cellular proteomics : MCP · 2026Article
- Interplay of CaThe Journal of physiology · 2026Article
- Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathways.Nucleic acids research · 2025Article
- The CENP-A chaperone complex spatially organizes centromeres.bioRxiv : the preprint server for biology · 2025Article
- IMPACTS OF DNA METHYLATION ON H2A.Z DEPOSITION AND NUCLEOSOME STABILITY.bioRxiv : the preprint server for biology · 2025Article
- Leading and lagging strand abasic sites differentially affect vertebrate replisome progression but involve analogous bypass mechanisms.Nucleic acids research · 2025Article
- Optimized Analysis of Proteins from Xenopus Oocytes and Embryos by Immunoblotting.Journal of visualized experiments : JoVE · 2025Article
- Supracellular contractility in Xenopus embryo epithelia regulated by extracellular ATP and the purinergic receptor P2Y2.Journal of cell science · 2025Article
- An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts.Journal of cell science · 2025Article
- Expanding Targeted Instrumentation for Discovery Applications: Complement Reporter Ion Quantification with a Quadrupole-Ion Trap Instrument.Journal of proteome research · 2025Article
- Transitions in the proteome and phospho-proteome during Xenopus laevis development.Developmental biology · 2025Article
- Spindle morphology changes between meiosis and mitosis driven by CK2 regulation of the Ran pathway.The Journal of cell biology · 2025Article
- Decoding protein phosphorylation during oocyte meiotic divisions using phosphoproteomics.eLife · 2025Article
- Article
117 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundMass spectrometry-based proteomics enables the global identification and quantification of proteins and their posttranslational modifications in complex biological samples. However, proteomic analysis requires a complete and accurate reference set of proteins and is therefore largely restricted to model organisms with sequenced genomes.
resultsHere, we demonstrate the feasibility of deep genome-free proteomics by using a reference proteome derived from heterogeneous mRNA data. We identify more than 11,000 proteins with 99% confidence from the unfertilized Xenopus laevis egg and estimate protein abundance with approximately 2-fold precision. Our reference database outperforms the provisional gene models based on genomic DNA sequencing and references generated by other methods. Surprisingly, we find that many proteins in the egg lack mRNA support and that many of these proteins are found in blood or liver, suggesting that they are taken up from the blood plasma, together with yolk, during oocyte growth and maturation, potentially contributing to early embryogenesis.
conclusionTo facilitate proteomics in nonmodel organisms, we make our platform available as an online resource that converts heterogeneous mRNA data into a protein reference set. Thus, we demonstrate the feasibility and power of genome-free proteomics while shedding new light on embryogenesis in vertebrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.