ArticleBMC developmental biology2014
Effects of dimethyl sulfoxide on asymmetric division and cytokinesis in mouse oocytes.
Article in BMC developmental biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Disparity between Post-thaw Survival and Functional Integrity of Vitrified-thawed Oocytes from Obese Mice.JBRA assisted reproduction · 2026Article
- Tankyrase activity is essential for asymmetric division and chromosome segregation in oocyte meiosis.Journal of advanced research · 2026Article
- Optimization of mouse oocyte vitrification through microencapsulation in sodium alginate hydrogel.Journal of assisted reproduction and genetics · 2025Article
- Oocytes Vitrification Using Automated Equipment Based on Microfluidic Chip.Annals of biomedical engineering · 2025Article
- Article
- Adverse PFAS effects on mouse oocyte in vitro maturation are associated with carbon-chain length and inclusion of a sulfonate group.Cell proliferation · 2023Article
- The Antioxidant Auraptene Improves Aged Oocyte Quality and Embryo Development in Mice.Antioxidants (Basel, Switzerland) · 2022Article
- Mouse oocyte vitrification with and without dimethyl sulfoxide: influence on cryo-survival, development, and maternal imprinted gene expression.Journal of assisted reproduction and genetics · 2021Article
- DMSO induces drastic changes in human cellular processes and epigenetic landscape in vitro.Scientific reports · 2019Article
- The cytotoxic effects of dimethyl sulfoxide in mouse preimplantation embryos: a mechanistic study.Theranostics · 2017Article
- Dimethyl Sulfoxide Perturbs Cell Cycle Progression and Spindle Organization in Porcine Meiotic Oocytes.PloS one · 2016Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances. DMSO has various biological and pharmacological activities; however, the effect of DMSO on mouse oocyte meiotic maturation remains unknown.
resultsIn DMSO-treated oocytes, we observed abnormal MII oocytes that contained large polar bodies, including 2-cell-like MII oocytes, during in vitro maturation. Oocyte polarization did not occur, due to the absence of actin cap formation and spindle migration. These features are among the primary causes of abnormal symmetric division; however, analysis of the mRNA expression levels of genes related to asymmetric division revealed no significant difference in the expression of these factors between the 3% DMSO-treated group and the control group. After each "blastomere" of the 2-cell-like MII stage oocytes was injected by one sperm head respectively, the oocytes still possessed the ability to extrude the second polar body from each "blastomere" and to begin cleavage. However, MII oocytes with large polar bodies developed to the blastocyst stage after intracytoplasmic sperm injection (ICSI). Furthermore, other permeable cryoprotectants, such as ethylene glycol and glycerol, also caused asymmetric division failure.
conclusionPermeable cryoprotectants, such as DMSO, ethylene glycol, and glycerol, affect asymmetric division. DMSO disrupts cytokinesis completion by inhibiting cortical reorganization and polarization. Oocytes that undergo symmetric division maintain the ability to begin cleavage after ICSI.
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