ArticleApplied and environmental microbiology2014
Iron triggers λSo prophage induction and release of extracellular DNA in Shewanella oneidensis MR-1 biofilms.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 72 citations in OpenAlex.
- Prophage Activation Triggers Extracellular Synthesis of DNA in Bacterial Biofilms via Rolling Circle Replication.Angewandte Chemie (International ed. in English) · 2026Article
- Participation of the Transcriptional Regulator Fur in Modulating Biofilm Formation of the Salmonid PathogenInternational journal of molecular sciences · 2026Article
- Systematic evaluation of phenotypic variations induced by prophages in a clinical isolate ofmSystems · 2025Article
- Regulation of iron homeostasis by Fur and atypical response regulator SsoR via derepressor-inhibitor oscillation inApplied and environmental microbiology · 2025Article
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- A transcription factor from the cryptic Escherichia coli Rac prophage controls both phage and host operons.Nucleic acids research · 2025Article
- The potential impact of iron supply on the development of starvedFrontiers in microbiology · 2025Article
- Shewanella oneidensis: Biotechnological Application of Metal-Reducing Bacteria.Advances in biochemical engineering/biotechnology · 2025Review
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- Identifying components of theJournal of bacteriology · 2024Article
- An autonomous plasmid as an inovirus phage satellite.Applied and environmental microbiology · 2024Article
- A hybrid transistor with transcriptionally controlled computation and plasticity.Nature communications · 2024Article
- Interspecies ecological competition rejuvenates decayed Geobacter electroactive biofilm.The ISME journal · 2024Article
- Phage SPO1 Protein Gp49 Is a Novel RNA Binding Protein That Is Involved in Host Iron Metabolism.International journal of molecular sciences · 2023Article
- Exposure of Shewanella oneidensis MR-1 to Sublethal Doses of Ionizing Radiation Triggers Short-Term SOS Activation and Longer-Term Prophage Activation.Applied and environmental microbiology · 2023Article
- Electron shuttle-dependent biofilm formation and biocurrent generation: Concentration effects and mechanistic insights.Frontiers in microbiology · 2023Article
- The Origin, Function, Distribution, Quantification, and Research Advances of Extracellular DNA.International journal of molecular sciences · 2022Review
- Analysis of the anaerobic digestion metagenome under environmental stresses stimulating prophage induction.Microbiome · 2022Article
- Deconstructing the Phage-Bacterial Biofilm Interaction as a Basis to Establish New Antibiofilm Strategies.Viruses · 2022Review
- Deciphering Active Prophages from Metagenomes.mSystems · 2022Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prophages are ubiquitous elements within bacterial chromosomes and affect host physiology and ecology in multiple ways. We have previously demonstrated that phage-induced lysis is required for extracellular DNA (eDNA) release and normal biofilm formation in Shewanella oneidensis MR-1. Here, we investigated the regulatory mechanisms of prophage λSo spatiotemporal induction in biofilms. To this end, we used a functional fluorescence fusion to monitor λSo activation in various mutant backgrounds and in response to different physiological conditions. λSo induction occurred mainly in a subpopulation of filamentous cells in a strictly RecA-dependent manner, implicating oxidative stress-induced DNA damage as the major trigger. Accordingly, mutants affected in the oxidative stress response (ΔoxyR) or iron homeostasis (Δfur) displayed drastically increased levels of phage induction and abnormal biofilm formation, while planktonic cells were not or only marginally affected. To further investigate the role of oxidative stress, we performed a mutant screen and identified two independent amino acid substitutions in OxyR (T104N and L197P) that suppress induction of λSo by hydrogen peroxide (H2O2). However, λSo induction was not suppressed in biofilms formed by both mutants, suggesting a minor role of intracellular H2O2 in this process. In contrast, addition of iron to biofilms strongly enhanced λSo induction and eDNA release, while both processes were significantly suppressed at low iron levels, strongly indicating that iron is the limiting factor. We conclude that uptake of iron during biofilm formation triggers λSo-mediated lysis of a subpopulation of cells, likely by an increase in iron-mediated DNA damage sensed by RecA.
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