ArticleApplied and environmental microbiology2014
The functional structure of central carbon metabolism in Pseudomonas putida KT2440.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 114 citations in OpenAlex.
- Simultaneous optimization of lignocellulosic sugar catabolism via systematic laboratory evolution under complex selection pressure.Nature communications · 2026Article
- Acetate as alternative carbon source for production of mono- and di-rhamnolipids in Pseudomonas putida KT2440.Microbial cell factories · 2026Article
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- Implementation of the synthetic arabinose 5-phosphate dependent glycolaldehyde assimilation (SAGA) pathway inFrontiers in bioengineering and biotechnology · 2026Article
- Glyceraldehyde-3-phosphate dehydrogenase homologs as bifunctional gatekeepers of metabolic segregation inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Role of Glyceraldehyde-3-Phosphate Dehydrogenase in 2-Ketogluconic Acid Industrial Production StrainFoods (Basel, Switzerland) · 2025Article
- Enhanced production of microbial levulinic acid through deletion of the levulinic acid transcriptional regulator (lvaR) in engineered Pseudomonas putida KT2440.Bioprocess and biosystems engineering · 2025Article
- Model of metabolism and gene expression predicts proteome allocation in Pseudomonas putida.NPJ systems biology and applications · 2025Article
- Modification of Glucose Metabolic Pathway to Enhance Polyhydroxyalkanoate Synthesis inCurrent issues in molecular biology · 2024Article
- GnuR Represses the Expression of Glucose and Gluconate Catabolism in Pseudomonas putida KT2440.Microbial biotechnology · 2024Article
- Enhanced biosynthesis of poly(3-hydroxybutyrate) in engineered strains of Pseudomonas putida via increased malonyl-CoA availability.Microbial biotechnology · 2024Article
- Physiology, fast and slow: bacterial response to variable resource stoichiometry and dilution rate.mSystems · 2024Article
- Machine learning analysis of RB-TnSeq fitness data predicts functional gene modules inmSystems · 2024Article
- Co-feeding enhances the yield of methyl ketones.Journal of industrial microbiology & biotechnology · 2023Article
- Integration of text mining and biological network analysis: Identification of essential genes in sulfate-reducing bacteria.Frontiers in microbiology · 2023Article
- Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis.Frontiers in bioengineering and biotechnology · 2022Review
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- Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress.The ISME journal · 2021Article
- Trehalose alleviates high-temperature stress in Pleurotus ostreatus by affecting central carbon metabolism.Microbial cell factories · 2021Article
- Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
What defines central carbon metabolism? The classic textbook scheme of central metabolism includes the Embden-Meyerhof-Parnas (EMP) pathway of glycolysis, the pentose phosphate pathway, and the citric acid cycle. The prevalence of this definition of central metabolism is, however, equivocal without experimental validation. We address this issue using a general experimental approach that combines the monitoring of transcriptional and metabolic flux changes between steady states on alternative carbon sources. This approach is investigated by using the model bacterium Pseudomonas putida with glucose, fructose, and benzoate as carbon sources. The catabolic reactions involved in the initial uptake and metabolism of these substrates are expected to show a correlated change in gene expressions and metabolic fluxes. However, there was no correlation for the reactions linking the 12 biomass precursor molecules, indicating a regulation mechanism other than mRNA synthesis for central metabolism. This result substantiates evidence for a (re)definition of central carbon metabolism including all reactions that are bound to tight regulation and transcriptional invariance. Contrary to expectations, the canonical Entner-Doudoroff and EMP pathways sensu stricto are not a part of central carbon metabolism in P. putida, as they are not regulated differently from the aromatic degradation pathway. The regulatory analyses presented here provide leads on a qualitative basis to address the use of alternative carbon sources by deregulation and overexpression at the transcriptional level, while rate improvements in central carbon metabolism require careful adjustment of metabolite concentrations, as regulation resides to a large extent in posttranslational and/or metabolic regulation.
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