ArticleApplied and environmental microbiology2014
Transcriptional regulator LsrB of Sinorhizobium meliloti positively regulates the expression of genes involved in lipopolysaccharide biosynthesis.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- An integrated Msr-antioxidase-host gene circuit maintains redox homeostasis in legume-rhizobium symbiosis.Frontiers in plant science · 2026Article
- Transcriptomic response ofFrontiers in microbiology · 2023Article
- Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies.Plants (Basel, Switzerland) · 2022Review
- A Lipopolysaccharide Synthesis GeneMicroorganisms · 2022Article
- Redox Regulation in Diazotrophic Bacteria in Interaction with Plants.Antioxidants (Basel, Switzerland) · 2021Review
- Article
- A central role for the transcriptional regulator VtlR in small RNA-mediated gene regulation in Agrobacterium tumefaciens.Scientific reports · 2020Article
- Riboregulation in Nitrogen-Fixing Endosymbiotic Bacteria.Microorganisms · 2020Review
- A high-throughput system to identify inhibitors ofProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Molecular Weapons Contribute to Intracellular Rhizobia Accommodation Within Legume Host Cell.Frontiers in plant science · 2019Review
- Involvement of Glutaredoxin and Thioredoxin Systems in the Nitrogen-Fixing Symbiosis between Legumes and Rhizobia.Antioxidants (Basel, Switzerland) · 2018Review
- Sinorhizobium meliloti Glutathione Reductase Is Required for both Redox Homeostasis and Symbiosis.Applied and environmental microbiology · 2018Article
- An account of evolutionary specialization: the AbcR small RNAs in the Rhizobiales.Molecular microbiology · 2018Review
- A LysR-family transcriptional regulator required for virulence in Brucella abortus is highly conserved among the α-proteobacteria.Molecular microbiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizobia induce nitrogen-fixing nodules on host legumes, which is important in agriculture and ecology. Lipopolysaccharide (LPS) produced by rhizobia is required for infection or bacteroid survival in host cells. Genes required for LPS biosynthesis have been identified in several Rhizobium species. However, the regulation of their expression is not well understood. Here, Sinorhizobium meliloti LsrB, a member of the LysR family of transcriptional regulators, was found to be involved in LPS biosynthesis by positively regulating the expression of the lrp3-lpsCDE operon. An lsrB in-frame deletion mutant displayed growth deficiency, sensitivity to the detergent sodium dodecyl sulfate, and acidic pH compared to the parent strain. This mutant produced slightly less LPS due to lower expression of the lrp3 operon. Analysis of the transcriptional start sites of the lrp3 and lpsCDE gene suggested that they constitute one operon. The expression of lsrB was positively autoregulated. The promoter region of lrp3 was specifically precipitated by anti-LsrB antibodies in vivo. The promoter DNA fragment containing TN11A motifs was bound by the purified LsrB protein in vitro. These new findings suggest that S. meliloti LsrB is associated with LPS biosynthesis, which is required for symbiotic nitrogen fixation on some ecotypes of alfalfa plants.
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