ArticleApplied and environmental microbiology2014
LacR is a repressor of lacABCD and LacT is an activator of lacTFEG, constituting the lac gene cluster in Streptococcus pneumoniae.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 33 citations in OpenAlex.
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- The Effect of Impaired Polyamine Transport on Pneumococcal Transcriptome.Pathogens (Basel, Switzerland) · 2021Article
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- The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression.Microorganisms · 2021Article
- Site-Specific Mutations of GalR Affect Galactose Metabolism in Streptococcus pneumoniae.Journal of bacteriology · 2020Article
- NADH-Mediated Gene Expression inFrontiers in microbiology · 2018Article
- Niacin-mediated Gene Expression and Role of NiaR as a Transcriptional Repressor ofFrontiers in cellular and infection microbiology · 2017Article
- Proteomic variation and diversity in clinical Streptococcus pneumoniae isolates from invasive and non-invasive sites.PloS one · 2017Article
- Methionine-mediated gene expression and characterization of the CmhR regulon inMicrobial genomics · 2016Article
- Cysteine-Mediated Gene Expression and Characterization of the CmbR Regulon inFrontiers in microbiology · 2016Article
- N-acetylglucosamine-Mediated Expression ofFrontiers in cellular and infection microbiology · 2016Article
- Carbon catabolite repression by seryl phosphorylated HPr is essential to Streptococcus pneumoniae in carbohydrate-rich environments.Molecular microbiology · 2015Article
- Sialic acid-mediated gene expression in Streptococcus pneumoniae and role of NanR as a transcriptional activator of the nan gene cluster.Applied and environmental microbiology · 2015Article
- A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae.Journal of visualized experiments : JoVE · 2015Article
- The Small Molecule DAM Inhibitor, Pyrimidinedione, Disrupts Streptococcus pneumoniae Biofilm Growth In Vitro.PloS one · 2015Article
- Maltose-Dependent Transcriptional Regulation of the mal Regulon by MalR in Streptococcus pneumoniae.PloS one · 2015Article
- Ascorbic acid-dependent gene expression in Streptococcus pneumoniae and the activator function of the transcriptional regulator UlaR2.Frontiers in microbiology · 2015Article
- Ni2+-Dependent and PsaR-Mediated Regulation of the Virulence Genes pcpA, psaBCA, and prtA in Streptococcus pneumoniae.PloS one · 2015Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comparison of the transcriptome of Streptococcus pneumoniae strain D39 grown in the presence of either lactose or galactose with that of the strain grown in the presence of glucose revealed the elevated expression of various genes and operons, including the lac gene cluster, which is organized into two operons, i.e., lac operon I (lacABCD) and lac operon II (lacTFEG). Deletion of the DeoR family transcriptional regulator lacR that is present downstream of the lac gene cluster revealed elevated expression of lac operon I even in the absence of lactose. This suggests a function of LacR as a transcriptional repressor of lac operon I, which encodes enzymes involved in the phosphorylated tagatose pathway in the absence of lactose or galactose. Deletion of lacR did not affect the expression of lac operon II, which encodes a lactose-specific phosphotransferase. This finding was further confirmed by β-galactosidase assays with PlacA-lacZ and PlacT-lacZ in the presence of either lactose or glucose as the sole carbon source in the medium. This suggests the involvement of another transcriptional regulator in the regulation of lac operon II, which is the BglG-family transcriptional antiterminator LacT. We demonstrate the role of LacT as a transcriptional activator of lac operon II in the presence of lactose and CcpA-independent regulation of the lac gene cluster in S. pneumoniae.
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