Evidence map›Paper›PMID 24951783›Full record

ArticleApplied and environmental microbiology2014

Microbial community degradation of widely used quaternary ammonium disinfectants.

Seungdae Oh, Zohre Kurt, Despina Tsementzi, Michael R Weigand, Minjae Kim, Janet K Hatt, Madan Tandukar, Spyros G Pavlostathis, Jim C Spain, Konstantinos T Konstantinidis

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 75 citations in OpenAlex.

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  12. Adaptation and Survival ofFrontiers in bioengineering and biotechnology · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Seungdae OhSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Zohre KurtSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Despina TsementziSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Michael R WeigandSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Minjae KimSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Janet K HattSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Madan TandukarSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Spyros G PavlostathisSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Jim C SpainSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Konstantinos T KonstantinidisSchool of Civil and Environmental Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA School of Biology, Georgia Institute of Technology, Atlanta, Georgia, USA Center for Bioinformatics and Computational Genomics, Georgia Institute of Technology, Atlanta, Georgia, USA kostas@ce.gatech.edu.
Georgia Institute of Technology · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Benzalkonium chlorides (BACs) are disinfectants widely used in a variety of clinical and environmental settings to prevent microbial infections, and they are frequently detected in nontarget environments, such as aquatic and engineered biological systems, even at toxic levels. Therefore, microbial degradation of BACs has important ramifications for alleviating disinfectant toxicity in nontarget environments as well as compromising disinfectant efficacy in target environments. However, how natural microbial communities respond to BAC exposure and what genes underlie BAC biodegradation remain elusive. Our previous metagenomic analysis of a river sediment microbial community revealed that BAC exposure selected for a low-diversity community, dominated by several members of the Pseudomonas genus that quickly degraded BACs. To elucidate the genetic determinants of BAC degradation, we conducted time-series metatranscriptomic analysis of this microbial community during a complete feeding cycle with BACs as the sole carbon and energy source under aerobic conditions. Metatranscriptomic profiles revealed a candidate gene for BAC dealkylation, the first step in BAC biodegradation that results in a product 500 times less toxic. Subsequent biochemical assays and isolate characterization verified that the putative amine oxidase gene product was functionally capable of initiating BAC degradation. Our analysis also revealed cooperative interactions among community members to alleviate BAC toxicity, such as the further degradation of BAC dealkylation by-products by organisms not encoding amine oxidase. Collectively, our results advance the understanding of BAC aerobic biodegradation and provide genetic biomarkers to assess the critical first step of this process in nontarget environments.

Indexed as

Gene Expression Regulation, BacterialMetagenomicsTranscriptomeAerobiosisBase SequenceBenzalkonium CompoundsBiodegradation, EnvironmentalCarbonCluster AnalysisDealkylationDisinfectantsDNA, BacterialGenetic MarkersModels, BiologicalPseudomonasRNA, AntisenseBenzalkonium CompoundsCarbonDisinfectantsDNA, BacterialGenetic MarkersRNA, AntisenseRNA, Ribosomal

Identifiers

PMID24951783
PMCPMC4178686
OpenAlexW2124800228

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.