ArticleProceedings of the National Academy of Sciences of the United States of America2014
EARLY BUD-BREAK 1 (EBB1) is a regulator of release from seasonal dormancy in poplar trees.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85 citing papers in PubMed, 179 citations in OpenAlex.
- PpERF27-PpCBF2/4 Module Integrates Low-Temperature and Ethylene Signals to Activate Abscisic Acid and DAM Pathways to Promote Bud Endodormancy in Peach.Plant, cell & environment · 2026Article
- Tissue specific mechanisms of tuber dormancy after 1,4-dimethylnaphthalene treatment in potato.Food chemistry. Molecular sciences · 2026Article
- Integrated BSA-Seq and WGCNA Analyses Reveal Candidate Genes Associated with Winter Bud Dormancy Maintenance in Fruit Mulberry (Current issues in molecular biology · 2025Article
- Natural variants ofScience advances · 2025Article
- Overexpression of Tempranillo-Like Proteins Promotes Dormancy Release in Poplar.Plant, cell & environment · 2025Article
- A transcriptional roadmap of the yearly growth cycle in Populus trees.The Plant cell · 2025Article
- PcMYBs responded to 6-BA to regulate PcCKXs to promote germination of primary rhizome buds of Polygonatum cyrtonema Hua.BMC plant biology · 2025Article
- Gene Regulatory Changes Associated With Phenological Transitions in an Ecologically Significant Tree Species.Plant-environment interactions (Hoboken, N.J.) · 2025Article
- Two aquaporins, LcPIP1;4 and LcPIP1;4a, cooperatively regulate the onset of dormancy of the terminal buds in evergreen perennial litchi (Horticulture research · 2025Article
- MADS-domain transcription factor AGAMOUS LIKE-9 participates in the gibberellin pathway to promote bud dormancy release of tree peony.Horticulture research · 2025Article
- Integrated analysis of the transcriptome and hormone metabolome elucidates the regulatory mechanisms governing walnut bud germination.BMC genomics · 2025Article
- Wake up: the regulation of dormancy release and bud break in perennial plants.Frontiers in plant science · 2025Review
- Genome-wide identification of GH17 family genes and their expression patterns associated with bud dormancy-regrowth regulation inFrontiers in plant science · 2025Article
- Molecular advances in bud dormancy in trees.Journal of experimental botany · 2024Review
- The AP2 transcription factor BARE RECEPTACLE regulates floral organogenesis via auxin pathways in woodland strawberry.The Plant cell · 2024Article
- Comparative transcriptome and functional analyses provide insights into the key factors regulating shoot regeneration in highbush blueberry.Horticulture research · 2024Article
- Integrated proteomic, transcriptomic, and metabolomic profiling reveals that the gibberellin-abscisic acid hub runs flower development in the Chinese orchidHorticulture research · 2024Article
- Signatures of local adaptation to current and future climate in phenology-related genes in natural populations of Quercus robur.BMC genomics · 2024Article
- Discovery of genes that positively affect biomass and stress associated traits in poplar.Frontiers in plant science · 2024Article
- Integrated metabolome and transcriptome analysis reveals potential mechanism during the bud dormancy transition ofFrontiers in plant science · 2024Article
25 more citing papers are in PubMed but not listed here.
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4 authors at 2 institutions in 1 country.
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Abstract
Trees from temperate latitudes transition between growth and dormancy to survive dehydration and freezing stress during winter months. We used activation tagging to isolate a dominant mutation affecting release from dormancy and identified the corresponding gene EARLY BUD-BREAK 1 (EBB1). We demonstrate through positioning of the tag, expression analysis, and retransformation experiments that EBB1 encodes a putative APETALA2/Ethylene responsive factor transcription factor. Transgenic up-regulation of the gene caused early bud-flush, whereas down-regulation delayed bud-break. Native EBB1 expression was highest in actively growing apices, undetectable during the dormancy period, but rapidly increased before bud-break. The EBB1 transcript was localized in the L1/L2 layers of the shoot meristem and leaf primordia. EBB1-overexpressing transgenic plants displayed enlarged shoot meristems, open and poorly differentiated buds, and a higher rate of cell division in the apex. Transcriptome analyses of the EBB1 transgenics identified 971 differentially expressed genes whose expression correlated with the EBB1 expression changes in the transgenic plants. Promoter analysis among the differentially expressed genes for the presence of a canonical EBB1-binding site identified 65 putative target genes, indicative of a broad regulatory context of EBB1 function. Our results suggest that EBB1 has a major and integrative role in reactivation of meristem activity after winter dormancy.
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