ArticlePlacenta2014
Banking placental tissue: an optimized collection procedure for genome-wide analysis of nucleic acids.
Article in Placenta, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Integrated analysis of transcriptomic datasets to identify placental biomarkers of spontaneous preterm birth.Placenta · 2022Pooled it
- Current approaches and developments in transcript profiling of the human placenta.Human reproduction update · 2020Pooled it
- Trial
- Determinants of Placental Versus Maternal Preeclampsia.Hypertension (Dallas, Tex. : 1979) · 2026Article
- Clinical and Molecular Differences of Hypertensive Disorders During Pregnancy.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- Comparison Between Two Methodologies of Sample Preservation for RNA Extraction in Naturally Delivered Ovine Placenta.Animals : an open access journal from MDPI · 2025Article
- Leveraging the placenta to advance neonatal care.Frontiers in pediatrics · 2023Review
- An optimized proteomics-based approach to estimate blood contamination and cellular heterogeneity of frozen placental tissue.Placenta · 2023Article
- Time-dependent changes in placental mRNA expression after delivery due to delayed specimen collection.American journal of reproductive immunology (New York, N.Y. : 1989) · 2021Article
- A new pipeline for clinico-pathological and molecular placental research utilizing FFPE tissues.Placenta · 2021Article
- Fat mass and obesity-associated (FTO) gene epigenetic modifications in gestational diabetes: new insights and possible pathophysiological connections.Acta diabetologica · 2021Article
- Adequate Placental Sampling for the Diagnosis and Characterization of Placental Infection by Zika Virus.Frontiers in microbiology · 2020Article
- In search of an evidence-based strategy for quality assessment of human tissue samples: report of the tissue Biospecimen Research Working Group of the Spanish Biobank Network.Journal of translational medicine · 2019Review
- Suitability of RNALater solution as a tissue-preserving reagent for immunohistochemical analysis.Histochemistry and cell biology · 2019Article
- Effect of prematurity on genome wide methylation in the placenta.BMC medical genetics · 2019Article
- Time course analysis of RNA quality in placenta preserved by RNAlater or flash freezing.American journal of reproductive immunology (New York, N.Y. : 1989) · 2017Article
- Article
- Preservation of Multiple Mammalian Tissues to Maximize Science Return from Ground Based and Spaceflight Experiments.PloS one · 2016Article
- Pre-Analytical Determination of the Effect of Extended Warm or Cold Ischaemia on RNA Stability in the Human Ileum Mucosa.PloS one · 2015Article
- Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
introductionBanking of high-quality placental tissue specimens will enable biomarker discovery and molecular studies on diseases involving placental dysfunction. Systematic studies aimed at developing feasible standardized methodology for placental collection in a typical clinical setting are lacking.
methodsTo determine the acceptable timeframe for placental collection, we collected multiple samples from first and third trimester placentas at serial timepoints in a 2-h window after delivery, simultaneously comparing the traditional snap-freeze technique to commercial solutions designed to preserve RNA (RNAlater™), and DNA (DNAgard(®)). The performance of RNAlater for preserving DNA was also tested. Nucleic acid quality was assessed by determining the RNA integrity number (RIN) and genome-wide microarray profiling for gene expression and DNA methylation.
resultsWe found that samples collected in RNAlater had higher and more consistent RINs compared to snap-frozen tissue. Similar RINs were obtained for tissue collected in RNAlater as large (1 cm(3)) and small (∼0.1 cm(3)) pieces. RNAlater appeared to better stabilize the time zero gene expression profile compared to snap-freezing for first trimester placenta. DNA methylation profiles remained quite stable over a 2 h time period after removal of the placenta from the uterus, with DNAgard being superior to other treatments. DISCUSSION AND
conclusionThe collection of placental samples in RNAlater and DNAgard is simple, and eliminates the need for liquid nitrogen or a freezer on-site. Moreover, the quality of the nucleic acids and the resulting data from samples collected in these preservation solutions is higher than samples collected using the snap-freeze method and easier to implement in busy clinical environments.
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Registered trials
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