ArticleReproductive biology and endocrinology : RB&E2014
Analysis of embryo morphokinetics, multinucleation and cleavage anomalies using continuous time-lapse monitoring in blastocyst transfer cycles.
Article in Reproductive biology and endocrinology : RB&E, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
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Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Does endometriosis inflict harm on embryos? A systematic review of embryo morphokinetics analysed by time lapse monitoring in women with endometriosis.Archives of gynecology and obstetrics · 2024Pooled it
- A double-blind randomized controlled trial investigating a time-lapse algorithm for selecting Day 5 blastocysts for transfer.Human reproduction (Oxford, England) · 2022Trial
- The endometrial preparation for frozen-thawed euploid blastocyst transfer: a prospective randomized trial comparing clinical results from natural modified cycle and exogenous hormone stimulation with GnRH agonist.Journal of assisted reproduction and genetics · 2016Trial
- c-Abl controls early embryonic morphogenesis through YAP/p73-dependent regulation of trophectoderm and inner cell mass lineages.Cell communication and signaling : CCS · 2026Article
- Factors influencing abnormal cleavage of early embryos: time-lapse imaging analysis of 138,178 normally fertilized embryos.Frontiers in endocrinology · 2026Article
- Comparative outcomes of day 4 versus day 5 embryo transfers by fertilization method.Frontiers in endocrinology · 2026Article
- Commentary: Association between pretransfer cleavage-stage blastomere dynamics and pregnancy outcomes in fresh single embryo transfer cycles: a retrospective cohort study.Frontiers in endocrinology · 2026Article
- Nuclear error phenotypes at the first mitosis predict cleavage-stage outcomes but not blastocyst competence.Frontiers in reproductive health · 2026Article
- Parental dietary patterns and assisted reproductive technology outcomes including embryo morphokinetics: rotterdam periconception cohort.Journal of assisted reproduction and genetics · 2025Article
- Non-Invasive Extracellular Vesicle Biomarkers in Endometriosis, Molecular Signatures Linking Pelvic Inflammation, Oocyte Quality, and IVF OutcomesCurrent issues in molecular biology · 2025Review
- Micro magnetic resonance spectroscopy for noninvasive metabolic screening of mammalian embryos and oocytes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Istanbul consensus update: a revised ESHRE/ALPHA consensus on oocyte and embryo static and dynamic morphological assessment†,‡.Human reproduction (Oxford, England) · 2025Article
- Could cleaved embryo morphology and morphokinetics be associated with prenatal and neonatal outcomes?Journal of assisted reproduction and genetics · 2025Article
- Nuclear error phenotypes in the two-cell embryo are correlated to blastocyst formation rate after assisted reproduction.Journal of assisted reproduction and genetics · 2025Article
- Delays in the final stages of fertilization are strongly associated with trichotomous cytokinesis and cleavage arrest.Journal of assisted reproduction and genetics · 2025Article
- Exploring key embryonic developmental morphokinetic parameters that affect clinical outcomes during the PGT cycle using time-lapse monitoring systems.BMC pregnancy and childbirth · 2024Article
- High proportion of zygotes with multiple pronuclei increase the embryo multinucleation rate during conventional IVF.Scientific reports · 2024Article
- Correlation between Human Embryo Morphokinetics Observed through Time-Lapse Incubator and Life Birth Rate.Journal of personalized medicine · 2024Article
- The mammalian sperm factor phospholipase C zeta is critical for early embryo division and pregnancy in humans and mice.Human reproduction (Oxford, England) · 2024Article
- A novel non-invasive embryo evaluation method (NICS-Timelapse) with enhanced predictive precision and clinical impact.Heliyon · 2024Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTime-lapse imaging combined with embryo morphokinetics may offer a non-invasive means for improving embryo selection. Data from clinics worldwide are necessary to compare and ultimately develop embryo classifications models using kinetic data. The primary objective of this study was to determine if there were kinetic differences between embryos with limited potential and those more often associated with in vitro blastocyst formation and/or implantation. We also wanted to compare putative kinetic markers for embryo selection as proposed by other laboratories to what we were observing in our own laboratory setting.
methodsKinetic data and cycle outcomes were retrospectively analyzed in patients age 39 and younger with 7 or more zygotes cultured in the Embryoscope. Timing of specific events from the point of insemination were determined using time-lapse (TL) imaging. The following kinetic markers were assessed: time to syngamy (tPNf), t2, time to two cells (c), 3c (t3), 4c ( t4), 5c (t5), 8c (t8), morula (tMor), start of blastulation (tSB); tBL, blastocyst (tBL); expanded blastocyst (tEBL). Durations of the second (cc2) and third (cc3) cell cycles, the t5-t2 interval as well as time to complete synchronous divisions s1, s2 and s3 were calculated. Incidence and impact on development of nuclear and cleavage anomalies were also assessed.
resultsA total of 648 embryos transferred on day 5 were analyzed. The clinical pregnancy and implantation rate were 72% and 50%, respectively. Morphokinetic data showed that tPNf, t2,t4, t8, s1, s2,s3 and cc2 were significantly different in embryos forming blastocysts (ET or frozen) versus those with limited potential either failing to blastulate or else forming poor quality blastocysts ,ultimately discarded. Comparison of embryo kinetics in cycles with all embryos implanting (KID+) versus no implantation (KID-) suggested that markers of embryo competence to implant may be different from ability to form a blastocyst. The incidence of multinucleation and reverse cleavage amongst the embryos observed was 25% and 7%, respectively. Over 40% of embryos exhibiting these characteristics did however form blastocysts meeting our criteria for freezing.
conclusionsThese data provide us with a platform with which to potentially enhance embryo selection for transfer.
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