Evidence map›Paper›PMID 24949660›Full record

ArticleEndocrinology2014

The vitamin D receptor (VDR) is expressed in skeletal muscle of male mice and modulates 25-hydroxyvitamin D (25OHD) uptake in myofibers.

Christian M Girgis, Nancy Mokbel, Kuan Minn Cha, Peter J Houweling, Myriam Abboud, David R Fraser, Rebecca S Mason, Roderick J Clifton-Bligh, Jenny E Gunton

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
106citing papers in PubMed, 2 pooled it
13.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

106 citing papers in PubMed, 2 syntheses or guidelines pooled it, 197 citations in OpenAlex.

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  11. Serum 25-Hydroxyvitamin D and Intact Parathyroid Hormone Influence Muscle Outcomes in Children and Adolescents.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2018
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  14. Associations of 25-Hydroxyvitamin D and 1,25-Dihydroxyvitamin D With Bone Mineral Density, Bone Mineral Density Change, and Incident Nonvertebral Fracture.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2015
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  16. Does 25(OH)D deficiency affect muscle elasticity, physical function, pain, and quality of life? A shear-wave elastography-based cross-sectional study.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2026
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46 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Christian M GirgisGarvan Institute of Medical Research (C.M.G., N.M., K.M.C., J.E.G.), Sydney, New South Wales, Australia 2010; Faculties of Medicine (C.M.G., M.A., R.S.M., R.J.C.-B., J.E.G.) and Veterinary Science (D.R.F.) University of Sydney, Sydney, New South Wales, Australia 2145; Bosch Institute (M.A., R.S.M.), University of Sydney, Sydney, New South Wales, Australia 2006; Murdoch Childrens Research Institute (P.J.H.), Melbourne, Victoria, Australia 3000; The Kolling Institute of Medical Research (R.J.C.-B.), Sydney, New South Wales, Australia 2065; Royal North Shore Hospital (R.J.C.-B.), Sydney, New South Wales, Australia 2065; Department of Endocrinology and Diabetes (J.E.G.), Westmead Hospital, Sydney, New South Wales, Australia 2145; and St Vincent's Clinical School (J.E.G.), University of New South Wales, Sydney, New South Wales, Australia 2010.
Nancy Mokbel
Kuan Minn Cha
Peter J Houweling
Myriam Abboud
David R Fraser
Rebecca S Mason
Roderick J Clifton-Bligh
Jenny E Gunton
Garvan Institute of Medical Research · AURobert Bosch (Australia) · AUThe University of Sydney · AUMurdoch Children's Research Institute · AUWestmead Hospital · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vitamin D deficiency is associated with a range of muscle disorders, including myalgia, muscle weakness, and falls. In humans, polymorphisms of the vitamin D receptor (VDR) gene are associated with variations in muscle strength, and in mice, genetic ablation of VDR results in muscle fiber atrophy and motor deficits. However, mechanisms by which VDR regulates muscle function and morphology remain unclear. A crucial question is whether VDR is expressed in skeletal muscle and directly alters muscle physiology. Using PCR, Western blotting, and immunohistochemistry (VDR-D6 antibody), we detected VDR in murine quadriceps muscle. Detection by Western blotting was dependent on the use of hyperosmolar lysis buffer. Levels of VDR in muscle were low compared with duodenum and dropped progressively with age. Two in vitro models, C2C12 and primary myotubes, displayed dose- and time-dependent increases in expression of both VDR and its target gene CYP24A1 after 1,25(OH)2D (1,25 dihydroxyvitamin D) treatment. Primary myotubes also expressed functional CYP27B1 as demonstrated by luciferase reporter studies, supporting an autoregulatory vitamin D-endocrine system in muscle. Myofibers isolated from mice retained tritiated 25-hydroxyvitamin D3, and this increased after 3 hours of pretreatment with 1,25(OH)2D (0.1 nM). No such response was seen in myofibers from VDR knockout mice. In summary, VDR is expressed in skeletal muscle, and vitamin D regulates gene expression and modulates ligand-dependent uptake of 25-hydroxyvitamin D3 in primary myofibers.

Indexed as

AnimalsBiological TransportHumansMaleMiceMice, Inbred C57BLMice, KnockoutMuscle, SkeletalMyofibrilsReceptors, CalcitriolVitamin DVitamin D Deficiency25-hydroxyvitamin DReceptors, CalcitriolVitamin D

Identifiers

PMID24949660
PMCPMC4207908
OpenAlexW2132958321

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.