Michael hydratase alcohol dehydrogenase or just alcohol dehydrogenase?

Verena Resch, Jianfeng Jin, Bi-Shuang Chen, Ulf Hanefeld

Open access · goldAbstract read

In one paragraph

Article in AMB Express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.6field-weighted citation impact, top 32% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

Enantioselective Michael addition of water.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Verena ReschBiocatalysis, Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft, 2628 BL, The Netherlands.

Jianfeng JinBiocatalysis, Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft, 2628 BL, The Netherlands.

Bi-Shuang ChenBiocatalysis, Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft, 2628 BL, The Netherlands.

Ulf HanefeldBiocatalysis, Department of Biotechnology, Delft University of Technology, Julianalaan 136, Delft, 2628 BL, The Netherlands.

Delft University of Technology · NL

Funding

Austrian Science Fund FWF J 3292

6 · The paper itself

Abstract

The Michael hydratase - alcohol dehydrogenase (MhyADH) from Alicycliphilus denitrificans was previously identified as a bi-functional enzyme performing a hydration of α,β-unsaturated ketones and subsequent oxidation of the formed alcohols. The investigations of the bi-functionality were based on a spectrophotometric assay and an activity staining in a native gel of the dehydrogenase. New insights in the recently discovered organocatalytic Michael addition of water led to the conclusion that the previously performed experiments to identify MhyADH as a bi-functional enzyme and their results need to be reconsidered and the reliability of the methodology used needs to be critically evaluated.

Indexed as

Alcohol dehydrogenaseHydrataseMichael additionα,β-Unsaturated carbonyl compounds

Identifiers

PMID24949265

DOI10.1186/s13568-014-0030-2

PMCPMC4052635

OpenAlexW2096533528

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.