ArticleEvidence-based complementary and alternative medicine : eCAM2014
Proteomic Identification of Nrf2-Mediated Phase II Enzymes Critical for Protection of Tao Hong Si Wu Decoction against Oxygen Glucose Deprivation Injury in PC12 Cells.
Article in Evidence-based complementary and alternative medicine : eCAM, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Spatial regulation effects of Taohong Siwu decoction on disordered lipids in a rat model of ischemic stroke assessed using mass spectrometry imaging.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Article
- Anti-inflammatory effects of Tao Hong Si Wu Tang in mice with lung cancer and chronic obstructive pulmonary disease.World journal of clinical oncology · 2024Article
- Proteomic Analysis Reveals that Di Dang Decoction Protects Against Acute Intracerebral Hemorrhage Stroke in Rats by Regulating S100a8, S100a9 Col1a1, and Col1a2.Neuropsychiatric disease and treatment · 2021Article
- Quantitative Proteomics Reveals the Beneficial Effects of Low Glucose on Neuronal Cell Survival in anFrontiers in cellular neuroscience · 2020Article
- Sulforaphane Attenuates H₂O₂-induced Oxidant Stress in Human Trabecular Meshwork Cells (HTMCs) via the Phosphatidylinositol 3-Kinase (PI3K)/Serine/Threonine Kinase (Akt)-Mediated Factor-E2-Related Factor 2 (Nrf2) Signaling Activation.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Differential proteomics for studying action mechanisms of traditional Chinese medicines.Chinese medicine · 2019Review
- Systems biology approaches in the study of Chinese herbal formulae.Chinese medicine · 2018Review
- DiDang Tang Inhibits Endoplasmic Reticulum Stress-Mediated Apoptosis Induced by Oxygen Glucose Deprivation and Intracerebral Hemorrhage Through Blockade of the GRP78-IRE1/PERK Pathways.Frontiers in pharmacology · 2018Article
- The Use of Omic Technologies Applied to Traditional Chinese Medicine Research.Evidence-based complementary and alternative medicine : eCAM · 2017Review
- Pretreatment with baicalin attenuates hypoxia and glucose deprivation-induced injury in SH-SY5Y cells.Chinese journal of integrative medicine · 2016Article
- Recent Advance in Applications of Proteomics Technologies on Traditional Chinese Medicine Research.Evidence-based complementary and alternative medicine : eCAM · 2015Review
- Baicalin protects against thrombin induced cell injury in SH-SY5Y cells.International journal of clinical and experimental pathology · 2015Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chinese herbal medicine formula Tao Hong Si Wu decoction (THSWD) is traditionally used in China for cerebrovascular diseases. However, the molecular mechanisms of THSWD associated with the cerebral ischemia reperfusion injury are largely unknown. The current study applied the two-dimensional gel electrophoresis-based proteomics to investigate the different protein profiles in PC12 cells with and without the treatment of THSWD. Twenty-six proteins affected by THSWD were identified by MALDI-TOF mass spectrometry. Gene ontology analysis showed that those proteins participated in several important biological processes and exhibited diverse molecular functions. In particular, six of them were found to be phase II antioxidant enzymes, which were regulated by NF-E2-related factor-2 (Nrf2). Quantitative PCR further confirmed a dose-dependent induction of the six phase II enzymes by THSWD at the transcription level. Moreover, the individual ingredients of THSWD were discovered to synergistically contribute to the induction of phase II enzymes. Importantly, THSWD's protection against oxygen-glucose deprivation-reperfusion (OGD-Rep) induced cell death was significantly attenuated by antioxidant response element (ARE) decoy oligonucleotides, suggesting the protection of THSWD may be likely regulated at least in part by Nrf2-mediated phase II enzymes. Thus, our data will help to elucidate the molecular mechanisms underlying the neuroprotective effect of THSWD.
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