ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2014
Evaluation of chromogenic media and seminested PCR in the identification of Candida species.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Phenotypic and Molecular Assessment ofPathogens (Basel, Switzerland) · 2026Article
- The role of biogenic aluminium oxide nanoparticles (AlScientific reports · 2025Article
- The impact of increasing non-albicans Candida trends on diagnostics in immunocompromised patients.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Article
- Oral health and the presence of infectious microorganisms in hospitalized patients: a preliminary observational study.Annals of medicine · 2022Observational
- Effectiveness of Oil-Based Denture Dentifrices-Organoleptic Characteristics, Physicochemical Properties and Antimicrobial Action.Antibiotics (Basel, Switzerland) · 2021Article
- Prevalence and antifungal drug resistance of nosocomialCurrent medical mycology · 2021Article
- High prevalence of asymptomatic nosocomial candiduria due toCurrent medical mycology · 2020Article
- Candidemia in pediatric burn patients: Risk factors and outcomes in a retrospective cohort study.Current medical mycology · 2020Article
- Molecular Identification of Candida Species in the Oral Microbiota of Individuals with Down Syndrome: A Case-Control Study.Mycopathologia · 2020Article
- Prevalence and antifungal susceptibility of Candida albicans causing vaginal discharge among pregnant women in Lebanon.BMC infectious diseases · 2020Article
- Biofilm-ProducingHIV/AIDS (Auckland, N.Z.) · 2020Article
- The effect of aqueous and ethanolic extract of Iranian propolis on Candida Albicans isolated from the mouth of patients with colorectal malignancy undergone chemotherapy: An in-vitro study.Caspian journal of internal medicine · 2020Article
- Current Aspects in the Biology, Pathogeny, and Treatment ofInfection and drug resistance · 2020Review
- Chromogenic medium versus PCR-RFLP in the speciation of Candida: a comparative study.BMC research notes · 2019Article
- Mycoses in northeastern Brazil: epidemiology and prevalence of fungal species in 8 years of retrospective analysis in Alagoas.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2019Article
- Emergence of Vulvovaginal Candidiasis among Lebanese Pregnant Women: Prevalence, Risk Factors, and Species Distribution.Infectious diseases in obstetrics and gynecology · 2019Article
- Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients.Revista do Instituto de Medicina Tropical de Sao Paulo · 2017Article
- A Decade of Development of Chromogenic Culture Media for Clinical Microbiology in an Era of Molecular Diagnostics.Clinical microbiology reviews · 2017Review
- Use of restriction fragment length polymorphism to identify Candida species, related to onychomycosis.Advanced biomedical research · 2015Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Identification of Candida cultured from various clinical specimens to the species level is increasingly necessary for clinical laboratories. Although sn PCR identifies the species within hours but its cost-effectiveness is to be considered. So there is always a need for media which help in the isolation and identification at the species level. The study aimed to evaluate the performance of different chromogenic media and to compare the effectiveness of the traditional phenotypic methods vs. seminested polymerase chain reaction (sn PCR) for identification of Candida species. One hundred and twenty seven Candida strains isolated from various clinical specimens were identified by conventional methods, four different chromogenic media and sn PCR. HiCrome Candida Differential and CHROMagar Candida media showed comparably high sensitivities and specificities in the identification of C. albicans, C. tropicalis, C. glabrata and C. krusei. CHROMagar Candida had an extra advantage of identifying all C. parapsilosis isolates. CHROMagar-Pal's medium identified C. albicans, C. tropicalis and C. krusei with high sensitivities and specificities, but couldn't identify C. glabrata or C. parapsilosis. It was the only medium that identified C. dubliniensis with a sensitivity and specificity of 100%. Biggy agar showed the least sensitivities and specificities. The overall concordance of the snPCR compared to the conventional tests including CHROMAgar Candida in the identification of Candida species was 97.5%. The use of CHROMAgar Candida medium is an easy and accurate method for presumptive identification of the most commonly encountered Candida spp.
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