ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2014
Aflatoxin detoxification by manganese peroxidase purified from Pleurotus ostreatus.
Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 125 citations in OpenAlex.
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- Microbial and enzymatic biodegradation of aflatoxins and ochratoxins: mechanisms, applications, and emerging innovations.Archives of microbiology · 2026Review
- Mycotoxin Removal and Transcriptional Response ofFoods (Basel, Switzerland) · 2025Article
- A Review of Research Progress on the Microbial or Enzymatic Degradation and Mechanism of Aflatoxin B1.Journal of microbiology and biotechnology · 2025Review
- Pleurotus spp.-an effective way in degradation mycotoxins? A comprehensive review.Mycotoxin research · 2025Review
- In Vivo Effectiveness ofMetabolites · 2025Article
- Mycotoxin Biodegradation byToxins · 2024Review
- Degradation of Toxins Derived from Foodborne Pathogens by Atmospheric-Pressure Dielectric-Barrier Discharge.International journal of molecular sciences · 2024Article
- Metabolites and degradation pathways of microbial detoxification of aflatoxins: a review.Mycotoxin research · 2024Review
- Characterization and mechanism of simultaneous degradation of aflatoxin BFrontiers in microbiology · 2024Article
- Isolation and Optimization of Aflatoxin BToxins · 2023Article
- SoilJournal of fungi (Basel, Switzerland) · 2023Review
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- Recent Research onFoods (Basel, Switzerland) · 2022Review
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- Invited review: Remediation strategies for mycotoxin control in feed.Journal of animal science and biotechnology · 2022Review
- Characterization of aFrontiers in microbiology · 2022Article
- Removal of Aflatoxin BToxins · 2021Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Manganese peroxidase (MnP) was produced from white rot edible mushroom Pleurotus ostreatus on the culture filtrate. The enzyme was purified to homogeneity using (NH4)2SO4 precipitation, DEAE-Sepharose and Sephadex G-100 column chromatography. The final enzyme activity achieved 81 U mL(-1), specific activity 78 U mg(-1) with purification fold of 130 and recovery 1.2% of the crude enzyme. SDS-PAGE indicated that the pure enzyme have a molecular mass of approximately 42 kDa. The optimum pH was between 4-5 and the optimum temperature was 25 °C. The pure MnP activity was enhanced by Mn(2+), Cu(2+), Ca(2+) and K(+) and inhibited by Hg(+2) and Cd(+2). H2O2 at 5 mM enhanced MnP activity while at 10 mM inhibited it significantly. The MnP-cDNA encoding gene was sequenced and determined (GenBank accession no. AB698450.1). The MnP-cDNA was found to consist of 497 bp in an Open Reading Frame (ORF) encoding 165 amino acids. MnP from P. ostreatus could detoxify aflatoxin B1 (AFB1) depending on enzyme concentration and incubation period. The highest detoxification power (90%) was observed after 48 h incubation at 1.5 U mL(-1) enzyme activities.
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